2-NBDG

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Chemical Structure| 186689-07-6 同义名 : NBD-Glucose
CAS号 : 186689-07-6
货号 : A422362
分子式 : C12H14N4O8
纯度 : 97%
分子量 : 342.262
MDL号 : MFCD23135882
存储条件：

Pure form Inert atmosphere,Store in freezer, under -20°C

In solvent -20°C:3-6个月-80°C:12个月
溶解度 :

H2O: 5 mg/mL(14.61 mM)，配合低频超声，并水浴加热至45℃助溶
动物实验配方：
生物活性
描述 2-NBDG, a fluorescent D-glucose analog, is a fluorescent indicator for monitoring glucose uptake into living cells. Ex: 467 nm; Em 542 nm. It has since been demonstrated in living mammalian cells that the uptake of 2-NBDG takes place through glucose transporters (GLUTs) in a concentration-, time- and temperature-dependent manner. A short-period application of 2-NBDG produced a remarkable increase in the fluorescence intensity in COS-1 cells over-expressing GLUT2, whereas the increase was barely detectable in mock-transfected cells. In mouse insulin-secreting clonal MIN6 cells, uptake was inhibited by cytochalasin B, a specific blocker for GLUTs, and by D-glucose in a dose-dependent manner[1]. Circulating breast cancer cells with increased uptake of fluorescent 2-NBDG were detected in mice bearing human breast cancer xenograft tumors by fluorescence imaging, suggesting clinical use of 2-NBDG as a tracer for fluorescence imaging of hypermetabolic circulating breast cancer cells[2]. 2-NBDG was continuously injected via the tail vein. Brain glucose metabolism was subsequently monitored by fluorescence imaging of 2-NBDG. The initial uptake rate of 2-NBDG at the injection site of 4-aminopyridine significantly exceeded that of the control injection site, which indicated local hypermetabolism induced by seizures. 2-NBDG can be used for localizing epileptic foci[3].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.92mL

0.58mL

0.29mL

14.61mL

2.92mL

1.46mL

29.22mL

5.84mL

2.92mL
参考文献

[1]Zou C, Wang Y, Shen Z. 2-NBDG as a fluorescent indicator for direct glucose uptake measurement. J Biochem Biophys Methods. 2005 Sep 30;64(3):207-15

[2]Cai H, Peng F. 2-NBDG fluorescence imaging of hypermetabolic circulating tumor cells in mouse xenograft model of breast cancer. J Fluoresc. 2013 Jan;23(1):213-20

[3]Tsytsarev V, Maslov KI, Yao J, Parameswar AR, Demchenko AV, Wang LV. In vivo imaging of epileptic activity using 2-NBDG, a fluorescent deoxyglucose analog. J Neurosci Methods. 2012 Jan 15;203(1):136-40