Methyl trans-p-Coumarate found in several plants, such as green onion (Allium cepa) or noni (Morinda citrifolia L.) leaves. Phytochemicals, viz., methyl trans-p-coumarate (AIC-1), methyl caffeate (AIC-2), syringic acid (AIC-3), and ursolic acid (UA), at different concentrations (500, 750, and 1000 ppm) were tested on spore germination of Alternaria alternata, Curvularia lunata, Fusarium moniliforme, F. pallidoroseum, and Helminthosporium sp. Significant growth inhibition was observed against all fungi except A. alternata which was found to be resistant to AIC-3[3]. Bifidobacterium animalis subsp. lactis, Lactobacillus gasseri, Lactobacillus acidophilus, Lactobacillus plantarum and Lactobacillus fermentum displayed activities against a broad substrate range (methyl caffeate, methyl trans-p-coumarate, chlorogenic acid as well as partially ethyl ferulate) [4]. The most active AIM-C (methanol extract chloroform) fraction was repeatedly chromatographed to yield four known compounds, methyl trans-p-coumarate (1), methyl caffeate (2), syringic acid (3) and pinellic acid (4). Cell viability assay of 1-4 against U87MG cells indicated 2 as most active (IC50 value of 8.2 μg/mL), whereas the other three compounds were much less active. Compounds 1-4 were non-toxic towards normal human cells (HEK-293) [5].
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