N-Dodecyl-β-D-maltoside, also known as Lauryl Maltoside, is a non-ionic detergent with strong affinity for alumina, titanium dioxide, and hematite. It facilitates the reactivation of various proteins and effectively stabilizes photoactive reaction center complexes (RCs), thereby preventing degradation of Rhodopseudomonas spheroides R-26 reaction center in solution. Additionally, it is utilized in the purification and stabilization of RNA polymerase and for the detection of protein-lipid interactions[1][2][3][4].
体外研究
N-Dodecyl-β-D-maltoside (0.02-10 mg/mL; 90 min) facilitates the reactivation of denatured adenosine deaminase, 3-phosphoglycerol phosphokinase, myokinase, and 3-hydroxysteroid dehydrogenase in the denatured enzyme dilution solution. The enzyme activity reaches its peak at 0.06 mg/mL, achieving 98% reactivation. However, it does not significantly promote the reactivation of phosphoglucomutase[1].
n-Dodecyl-β-D-maltoside 细胞实验
Cell Line
Concentration
Treated Time
Description
References
MCF7 cells
0.015%
1 hour
Evaluate the effect of DDM on MCF7 cell proteomics, results showed DDM significantly improved protein detection sensitivity and quantity
Commun Biol. 2021 Mar 1;4(1):265
MCF10A cells
0.015%
1 hour
Evaluate the effect of DDM on single-cell proteomics, results showed DDM significantly improved protein detection sensitivity and quantity
Commun Biol. 2021 Mar 1;4(1):265
Aquifex aeolicus leucine transporter (LeuT)
0.2 wt%, or 0.04 wt%
12 days
Evaluate the effect of DDM on LeuT stability. At CMC+0.04 wt% concentration, DDM showed a gradual decrease in Leu binding activity; at CMC+0.2 wt% concentration, DDM had lower initial activity but recovered over time. MTGs were superior to DDM in long-term stability.
ACS Chem Biol. 2020 Jun 19;15(6):1697-1707
Β1-adrenergic receptor (β1AR)
~60 µM
15 min incubation at room temperature, then kept at 4°C
Investigate the effects of CHS and sodium cholate on the structural and oligomerization preferences of β1AR. CHS binds specifically to the β1AR monomer at a site close to the Cys163-R1 spin label, stabilizing the monomeric form; sodium cholate promotes β1AR dimerization.
Proc Natl Acad Sci U S A. 2023 Feb 14;120(7):e2221036120
LH3 protein
0.02%
30 minutes
Investigate the effect of DDM on the spectral features of the B820 BChl a Qy band in LH3 protein, results showed DDM caused a redshift of the B820 Qy band accompanied by hyperchromism
ACS Omega. 2022 Aug 11;7(33):29231-29235
Maize mesophyll thylakoids
1% (w/V)
30 minutes
Detection of large photosystem I complexes, PSI complexes with different light-harvesting complex (LHC) complements were more abundant in mesophyll thylakoids of lincomycin treated maize.
Plant Methods. 2022 Mar 3;18(1):23
Maize bundle sheath thylakoids
1% (w/V)
30 minutes
Detection of large photosystem I complexes, several PSI-NDH-like megacomplexes were discernible in maize bundle sheath thylakoids.
Plant Methods. 2022 Mar 3;18(1):23
FreeStyle293-F cells
0.8%
30 minutes
Used to solubilize ABCG1-GFP and analyze its molecular size and monodispersity by fluorescence size exclusion chromatography
J Lipid Res. 2013 Feb;54(2):496-502
MCF-10A
0.5%
55 minutes
Used to solubilize exosomal proteins for enhanced mass spectrometry detection. DDM was more effective than other detergents, identifying 381 proteins.
Sci Rep. 2020 Aug 11;10(1):13572
MDA-MB-231
0.5%
55 minutes
Used to solubilize exosomal proteins for enhanced mass spectrometry detection. DDM was more effective than other detergents, identifying 986 proteins.
Sci Rep. 2020 Aug 11;10(1):13572
Human β2 adrenergic receptor (β2AR)
0.2 wt%
6 days
Evaluate the effect of DDM on β2AR stability. DDM rapidly reduced receptor activity to ~10% of initial activity within two days. In contrast, MTGs were significantly more effective than DDM in long-term stability.
ACS Chem Biol. 2020 Jun 19;15(6):1697-1707
PC9 cells
0.01%
Overnight
Reduce protein/peptide adsorptive losses on tube surfaces
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