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聚肌苷-胞酸 /Polyinosinic-polycytidylic acid {[allProObj[0].p_purity_real_show]}

货号:A719383 同义名: 聚胞苷酸 / Poly(I:C)

Polyinosinic acid-polycytidylic acid is a TLR3 agonist and an immunostimulant. It is used in the form of its sodium salt to simulate viral infections.

Polyinosinic-polycytidylic acid 化学结构 CAS号:24939-03-5
Polyinosinic-polycytidylic acid 化学结构
CAS号:24939-03-5
Polyinosinic-polycytidylic acid 3D分子结构
CAS号:24939-03-5
Polyinosinic-polycytidylic acid 化学结构 CAS号:24939-03-5
Polyinosinic-polycytidylic acid 3D分子结构 CAS号:24939-03-5
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Polyinosinic-polycytidylic acid 纯度/质量文件 产品仅供科研

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Polyinosinic-polycytidylic acid 生物活性

描述 Toll-like receptor-3 (TLR-3) belong to the Toll-like receptors family, it is mainly expressed on immune cells where it senses pathogen-associated molecular patterns and initiates innate immune response[3]. Polyinosinic-polycytidylic acid [poly (I:C)] can act as a dsRNA molecule and interacts with TLR-3 of microglia cells to evoke the immune system, thus mimicking the viral infection. Early life exposure to poly (I:C) has a long-term effect and impairs learning and memory, probably through TNF-α mediated neuroinflammation and alteration in the expression of memory associated genes in frontal cortex and hippocampus of rats[4]. Poly (I:C) exclusively stimulated the expression of interferon (IFN) β, IFNλ1, and the IFN-stimulated gene OAS2. Poly (I:C) also significantly increased secretion of elafin, CCL20, and RANTES. Secretions from poly (I:C)-treated fibroblasts had both greater anti-HIV activity and induced higher levels of CD4 + T cell chemotaxis than those from LPS-treated cells[5].

Polyinosinic-polycytidylic acid 临床研究

NCT号 适应症或疾病 临床期 招募状态 预计完成时间 地点
NCT01245673 Myeloma Phase 2 Active, not recruiting August 2018 United States, Maryland ... 展开 >> University of Maryland Greenebaum Cancer Center Baltimore, Maryland, United States, 21201 United States, Pennsylvania Abramson Cancer Center of the University of Pennsylvania Philadelphia, Pennsylvania, United States, 19104 收起 <<
NCT02425306 Melanoma Phase 1 Phase 2 Terminated(Slow accrual to par... 展开 >>t 2 of the study. Accrual to part 1 is complete.) 收起 << - United States, Virginia ... 展开 >> Cancer Center at the University of Virginia Charlottesville, Virginia, United States, 22908 收起 <<
NCT03121677 Follicular Lymphoma Phase 1 Recruiting January 31, 2028 United States, Missouri ... 展开 >> Washington University School of Medicine Recruiting Saint Louis, Missouri, United States, 63110 Contact: Todd A Fehinger, M.D., Ph.D.    314-362-5654    tfehnige@wustl.edu    Principal Investigator: Todd A Fehniger, M.D., Ph.D.          Sub-Investigator: Neha Mehta-Shah, M.D.          Sub-Investigator: Jeffrey Ward, M.D., Ph.D.          Sub-Investigator: Nancy L Bartlett, M.D.          Sub-Investigator: Brad Kahl, M.D.          Sub-Investigator: Amanda Cashen, M.D.          Sub-Investigator: Armin Ghobadi, M.D.          Sub-Investigator: John DiPersio, M.D., Ph.D.          Sub-Investigator: William E Gillanders, M.D.          Sub-Investigator: Malachi Griffith, Ph.D.          Sub-Investigator: Obi Griffith, Ph.D.          Sub-Investigator: Max Artyomov, Ph.D. 收起 <<

Polyinosinic-polycytidylic acid 参考文献

[1]Spranger S, Javorovic M, et al. Generation of Th1-polarizing dendritic cells using the TLR7/8 agonist CL075. J Immunol. 2010;185(1):738-47.

[2]Robbins MA, Maksumova L, et al. Nuclear factor-kappaB translocation mediates double-stranded ribonucleic acid-induced NIT-1 beta-cell apoptosis and up-regulates caspase-12 and tumor necrosis factor receptor-associated ligand (TRAIL). Endocrinology. 2003 Oct;144(10):4616-25.

[3]Bianchi F, Pretto S, Tagliabue E, Balsari A, Sfondrini L. Exploiting poly(I:C) to induce cancer cell apoptosis. Cancer Biol Ther. 2017 Oct 3;18(10):747-756. doi: 10.1080/15384047.2017.1373220. Epub 2017 Sep 7. PMID: 28881163; PMCID: PMC5678690.

[4]Baghel MS, Singh B, Dhuriya YK, Shukla RK, Patro N, Khanna VK, Patro IK, Thakur MK. Postnatal exposure to poly (I:C) impairs learning and memory through changes in synaptic plasticity gene expression in developing rat brain. Neurobiol Learn Mem. 2018 Nov;155:379-389. doi: 10.1016/j.nlm.2018.09.005. Epub 2018 Sep 6. PMID: 30195050.

[5]Patel MV, Shen Z, Wira CR. Poly (I:C) and LPS induce distinct immune responses by ovarian stromal fibroblasts. J Reprod Immunol. 2018 Jun;127:36-42. doi: 10.1016/j.jri.2018.05.001. Epub 2018 May 9. PMID: 29758486; PMCID: PMC5991091.

Polyinosinic-polycytidylic acid 实验方案

计算器
存储液制备 1mg 5mg 10mg

1 mM

5 mM

10 mM

1.49mL

0.30mL

0.15mL

7.45mL

1.49mL

0.74mL

14.89mL

2.98mL

1.49mL

Polyinosinic-polycytidylic acid 技术信息

CAS号24939-03-5
分子式(C10H13N4O8P)x.(C9H14N3O8P)x
分子量
别名 聚胞苷酸 ;Poly(I:C)
运输蓝冰
存储条件

液体 -20°C:3-6个月-80°C:12个月

粉末 Inert atmosphere,Store in freezer, under -20°C

溶解度

H2O: 50 mg/mL,配合低频超声助溶

动物实验配方
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