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O4I2 {[allProObj[0].p_purity_real_show]}

货号:A221899

O4I2 is an Oct3/4 inducer which can induce expression of pluripotent-associated genes.

O4I2 化学结构 CAS号:165682-93-9
O4I2 化学结构
CAS号:165682-93-9
O4I2 3D分子结构
CAS号:165682-93-9
O4I2 化学结构 CAS号:165682-93-9
O4I2 3D分子结构 CAS号:165682-93-9
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O4I2 纯度/质量文件 产品仅供科研

货号:A221899 标准纯度: {[allProObj[0].p_purity_real_show]}
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O4I2 生物活性

描述 Oct3/4 (Octamer-binding transcription factor 4), also referred to POU5F1, Oct4A, Oct3, or Oct4, is a member of the POU family of transcription factors1 expressed specifically in pluripotent cells, including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), it plays important role in regulation of the cellular pluripotency core network[2]. O4I2, an azaindole derivative, was reported to enhance Oct3/4 activation. AZ study showed that O4I2 demonstrated the highest Oct3/4 activation in HEK-Oct3/4 reporter cells in 3.5-fold at 20 μM after 72 h of treatment; After 72 h treatment with 20 μM in HEK293 cells, the immunoblot results revealed a clear accumulation of Oct3/4 and an increased level of Nanog protein, and analysis by qRTPCR suggested a significant induction of stemness-associated genes after stimulation with O4I2, including Oct3/4, Nanog, and Sox2, while the level of Lin28 remained unaffected and Rex1 was even reduced. In human primary skin fibroblasts (HFs), qRT-PCR analysis detected a marked increase of Oct3/ 4 expression, the induction of Lin28 and Rex1 and a moderate activation of Nanog after 48 h treatment with 20 μM O4I2. Moreover, immunocytochemistry detected coexpression of Oct3/4 and Sox2 as well as Nanog and Klf4 in HF cells treated with O4I2 for 5 days. O4I2 can also promote Oct3/4 accumulation in embryonal carcinoma NCCIT cells (NCCIT-Oct3/4) and HeLa cells [3].
作用机制 O4I2 promotes Oct3/4 expression by activating its promoter site but also stabilize the Oct3/4 protein, which regulates of the cellular pluripotency core network[3].

O4I2 参考文献

[1]Cheng X, Yoshida H, et al. Ethyl 2-((4-Chlorophenyl)amino)thiazole-4-carboxylate and Derivatives Are Potent Inducers of Oct3/4. J Med Chem. 2015 Aug 13;58(15):5742-50.

[2]Oct4

[3]Cheng X, Yoshida H, Raoofi D, Saleh S, Alborzinia H, Wenke F, Göhring A, Reuter S, Mah N, Fuchs H, Andrade-Navarro MA, Adjaye J, Gul S, Utikal J, Mrowka R, Wölfl S. Ethyl 2-((4-Chlorophenyl)amino)thiazole-4-carboxylate and Derivatives Are Potent Inducers of Oct3/4. J Med Chem. 2015 Aug 13;58(15):5742-50. doi: 10.1021/acs.jmedchem.5b00226. Epub 2015 Jul 23.

O4I2 实验方案

计算器
存储液制备 1mg 5mg 10mg

1 mM

5 mM

10 mM

3.54mL

0.71mL

0.35mL

17.68mL

3.54mL

1.77mL

35.37mL

7.07mL

3.54mL

O4I2 技术信息

CAS号165682-93-9
分子式C12H11ClN2O2S
分子量 282.746
别名
运输蓝冰
存储条件

液体 -20°C:3-6个月-80°C:12个月

粉末 Keep in dark place,Inert atmosphere,Room temperature

溶解度

DMSO: 40 mg/mL(141.47 mM),注意:DMSO长时间开封后,会吸水并导致溶解能力下降,请避免使用长期开封的DMSO

动物实验配方
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