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Protopanaxdiol

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Chemical Structure| 30636-90-9 同义名 : 20 (S)-原人参二醇 ;(20S)-Protopanaxadiol;20-Epiprotopanaxadiol;20S-Protopanaxadiol;20(S)-Protopanaxadiol;20(S)-APPD
CAS号 : 30636-90-9
货号 : A916196
分子式 : C30H52O3
纯度 : 98%
分子量 : 460.732
MDL号 : MFCD12032048
存储条件：

粉末 Sealed in dry,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 50 mg/mL(108.52 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 (20S)-Protopanaxadiol (20-Epiprotopanaxadiol) is an aglycon metabolic derivative of the protopanaxadiol-type ginseng saponin-apoptosis inducer. For the SF188 cells, (20S)-Protopanaxadiol activated caspases-3, -8, -7, and -9 within 3 h and induced rapid apoptosis, which could be partially inhibited by a general caspase blocker and completely abolished when the caspase blocker was used in combination with an antioxidant[3]. 20S-protopanaxadiol (PPD) showed the most potent cytotoxic activity in both LX-2 cells and primary activated HSCs (hepatic stellate cells) . In addition, 20S-PPD concentration-dependently increased the phosphorylation of AMPK, and compound C prevented 20S-PPD-induced cytotoxicity and mitochondrial dysfunction. Moreover, 20S-PPD increased the phosphorylation of liver kinase B1 (LKB1), an upstream kinase of AMPK. Likewise, transfection of LX-2 cells with LKB1 siRNA reduced the cytotoxic effect of 20S-PPD[4]. PPD (>30 μM) suppressed cell proliferation by arresting cell cycle in G0/1 phase and triggering cells apoptosis as shown by cell viability assay, flow cytometry analysis and colony formation assay, whereas lower dose of PPD (<20 μM) decreased metastatic potential of MDA-MB-231 and SUM159 cells through direct inhibition of cell adhesion, motility and invasiveness[5].
细胞研究
细胞系 浓度 检测类型 检测时间 活动说明 数据源
COLO205 cells Cytotoxicity assay 72 h Cytotoxicity against human COLO205 cells after 72 hrs by MTT assay 21216145
Hep3B cells Cytotoxicity assay 72 h Cytotoxicity against human Hep3B cells after 72 hrs by MTT assay 22342101
HepG2 cells Cytotoxicity assay 72 h Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay 21216145
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.17mL

0.43mL

0.22mL

10.85mL

2.17mL

1.09mL

21.70mL

4.34mL

2.17mL
参考文献

[1]Wanderi C, Kim E, et al. Ginsenoside 20(S)-Protopanaxadiol Suppresses Viability of Human Glioblastoma Cells via Down-regulation of Cell Adhesion Proteins and Cell-cycle Arrest. Anticancer Res. 2016 Mar;36(3):925-32.

[2]Oh HA, Kim DE, Choi HJ, Kim NJ, Kim DH. Anti-stress effects of 20(S)-protopanaxadiol and 20(S)-protopanaxatriol in immobilized mice. Biol Pharm Bull. 2015;38(2):331-5.

[3]Liu GY, Bu X, Yan H, Jia WW. 20S-protopanaxadiol-induced programmed cell death in glioma cells through caspase-dependent and -independent pathways. J Nat Prod. 2007 Feb;70(2):259-64

[4]Park SM, Jung EH, Kim JK, Jegal KH, Park CA, Cho IJ, Kim SC. 20S-Protopanaxadiol, an aglycosylated ginsenoside metabolite, induces hepatic stellate cell apoptosis through liver kinase B1-AMP-activated protein kinase activation. J Ginseng Res. 2017 Jul;41(3):392-402

[5]Peng B, He R, Xu Q, Yang Y, Hu Q, Hou H, Liu X, Li J. Ginsenoside 20(S)-protopanaxadiol inhibits triple-negative breast cancer metastasis in vivo by targeting EGFR-mediated MAPK pathway. Pharmacol Res. 2019 Apr;142:1-13