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Cephalotaxlen

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Chemical Structure| 24316-19-6 同义名 : (-)-Cephalotaxine;ZINC19795976;(-)-Cephalotaxine, Alkaloid A from Cephalotaxus, Cephalotaxine;NSC 245454;NSC 128487;(–)-Cephalotaxine;Cephalotaxine
CAS号 : 24316-19-6
货号 : A863555
分子式 : C18H21NO4
纯度 : 98%
分子量 : 315.364
MDL号 : MFCD00063372
存储条件：

粉末 Inert atmosphere,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 25 mg/mL(79.27 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 Cephalotaxlen is an alkaloid that can be isolated from Cephalotaxus drupacea, with antileukemic and antiviral activities[3]. CET (Cephalotaxine) activated the mitochondrial apoptosis pathway by reducing the mitochondrial membrane potential, downregulating anti-apoptotic Bcl-2 protein and upregulating pro-apoptotic Bak protein. CET blocked the fluorescence colocation of MitoTracker Green and LysoTracker Red and upregulated the level of LC3-II and p62, which indicated that autophagy flow was impaired. CET could impair lysosomal acidification and block autophagy flow. Finally, inhibiting autophagy flow could aggravate apoptosis of HL-60 cells induced by CET[4]. Cephalotaxine was effective in inhibiting ZIKV (Zika virus) activity in vitro (i.e., in Vero and A549 cell lines). CET (Cephalotaxine) was found to decrease ZIKV RNA and viral protein expression, inhibit ZIKV replication, and inhibit ZIKV mRNA/protein production[5].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

3.17mL

0.63mL

0.32mL

15.85mL

3.17mL

1.59mL

31.71mL

6.34mL

3.17mL
参考文献

[1]Hameed A, Blake AJ, Hayes CJ. A second generation formal synthesis of (-)-cephalotaxine. J Org Chem. 2008 Oct 17;73(20):8045-8.

[2]Wang DZ, Ma GE, Xu RS. Studies on the alkaloids of Cephalotaxus. IX. Semi-synthesis of cephalotaxine esters and their anti-leukemic activity Yao Xue Xue Bao. 1992;27(3):178-84.

[3]Wang DZ, Ma GE, Xu RS. [Studies on the alkaloids of Cephalotaxus. IX. Semi-synthesis of cephalotaxine esters and their anti-leukemic activity]. Yao Xue Xue Bao. 1992;27(3):178-84. Chinese

[4]Liu T, Guo Q, Zheng S, Liu Y, Yang H, Zhao M, Yao L, Zeng K, Tu P. Cephalotaxine Inhibits the Survival of Leukemia Cells by Activating Mitochondrial Apoptosis Pathway and Inhibiting Autophagy Flow. Molecules. 2021 May 18;26(10):2996

[5]Lai ZZ, Ho YJ, Lu JW. Cephalotaxine inhibits Zika infection by impeding viral replication and stability. Biochem Biophys Res Commun. 2020 Feb 19;522(4):1052-1058