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Oxidopamine hydrobromide

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Chemical Structure| 636-00-0 同义名 : 6-Hydroxydopamine hydrobromide;6-OHDA hydrobromide;2,4,5-Trihydroxyphenethylamine;Oxidopamine;6-hydroxy Dopamine;6-OHDA (hydrobromide);6-OHDA
CAS号 : 636-00-0
货号 : A818244
分子式 : C8H12BrNO3
纯度 : 98%
分子量 : 250.09
MDL号 : MFCD00012894
存储条件：

粉末 Inert atmosphere,Store in freezer, under -20°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 50 mg/mL(199.93 mM)，配合低频超声，并水浴加热至45℃助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO

H2O: 20 mg/mL(79.97 mM)，配合低频超声助溶
动物实验配方：
生物活性
描述 Oxidopamine hydrobromide (6-OHDA hydrobromide), an antagonist of the neurotransmitter dopamine, is a widely used neurotoxin that selectively destroys dopaminergic neurons. 6-OHDA-induced apoptosis of PC12 cells was initiated by superoxide generation followed by caspase cascade activation, which was associated with the suppressed Akt phosphorylation and increased p38 phosphorylation[3]. Moreover, the reduction in proteasomal activity by 6-OHDA was attenuated in SH-SY5Y cells pretreated with 1 μM CA(carnosic acid) [4]. Buspirone improves the anti-cataleptic effect of L-DOPA in a 6-OHDA-induced animal model of PD through the activation of 5-HT(1A) receptors[5].
临床研究
NCT号 适应症或疾病 临床期 招募状态 预计完成时间 地点
NCT01108029 Parkinson's Disease ... 展开 >> Gait Disorders, Neurologic 收起 << Phase 4 Completed - France ... 展开 >> Devos Lille, France, 59037 收起 <<
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

4.00mL

0.80mL

0.40mL

19.99mL

4.00mL

2.00mL

39.99mL

8.00mL

4.00mL
参考文献

[1]Fujita H, Ogino T, et al. Cell-permeable cAMP analog suppresses 6-hydroxydopamine-induced apoptosis in PC12 cells through the activation of the Akt pathway. Brain Res. 2006 Oct 3;1113(1):10-23.

[2]Soto-Otero R, Mendez-Alvarez E, et al. Autoxidation and neurotoxicity of 6-hydroxydopamine in the presence of some antioxidants: potential implication in relation to the pathogenesis of Parkinson's disease. J Neurochem. 2000 Apr;74(4):1605-12.

[3]Fujita H, Ogino T, Kobuchi H, Fujiwara T, Yano H, Akiyama J, Utsumi K, Sasaki J. Cell-permeable cAMP analog suppresses 6-hydroxydopamine-induced apoptosis in PC12 cells through the activation of the Akt pathway. Brain Res. 2006 Oct 3;1113(1):10-23

[4]Lin CY, Tsai CW, Tsai CW. Carnosic acid protects SH-SY5Y cells against 6-hydroxydopamine-induced cell death through upregulation of parkin pathway. Neuropharmacology. 2016 Nov;110(Pt A):109-117

[5]Mahmoudi J, Nayebi AM, Samini M, Reyhani-Rad S, Babapour V. Buspirone improves the anti-cataleptic effect of levodopa in 6-hydroxydopamine-lesioned rats. Pharmacol Rep. 2011;63(4):908-14