产品说明书
DiZPK
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同义名 : | - |
| CAS号 : | 1337883-32-5 | |
| 货号 : | A791385 | |
| 分子式 : | C12H23N5O3 | |
| 纯度 : | 98% | |
| 分子量 : | 285.343 | |
| MDL号 : | MFCD28900721 | |
| 存储条件： |
粉末 Keep in dark place,Inert atmosphere,Store in freezer, under -20°C 液体 -20°C:3-6个月-80°C:12个月 |
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| 溶解度 : |
H2O: 5 mg/mL(17.52 mM)，配合低频超声，并调节pH至7 |
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| 动物实验配方： |
| 生物活性 | |||
|---|---|---|---|
| 描述 | Protein photocrosslinking, via the introduction of non-natural amino acids, has emerged as a superior strategy for the covalent trapping of transient protein-protein interactions in vivo, which is generally not feasible using other methodologies. This method is particularly suitable for detecting periplasmic proteins interacting with HdeA at a low pH. DiZPK, an alkyl diazirine–containing unnatural amino acid, is a new photocrosslinker for identifying direct protein-protein interactions in living prokaryotic and eukaryotic cells[2]. | ||
| 作用机制 | Introduction of DiZPK at two dimer-interface sites, Phe35 and Ala38, yielded a crosslinking efficiency[2]. | ||
| 实验方案 | |||
|---|---|---|---|
| 1mg | 5mg | 10mg | |
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1 mM 5 mM 10 mM |
3.50mL 0.70mL 0.35mL |
17.52mL 3.50mL 1.75mL |
35.05mL 7.01mL 3.50mL |
| 参考文献 |
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