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AY 9944

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Chemical Structure| 366-93-8 同义名 : AY 9944 dihydrochloride
CAS号 : 366-93-8
货号 : A758319
分子式 : C22H30Cl4N2
纯度 : 98%
分子量 : 464.299
MDL号 : MFCD00034973
存储条件：

粉末 Inert atmosphere,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 4 mg/mL(8.62 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO

H2O: 5 mg/mL(10.77 mM)，配合低频超声助溶
动物实验配方：
生物活性
描述 AY 9944 is a specific cholesterol biosynthesis inhibitor that inhibits 7-dehydrocholesterol Δ7 reductase (DHCR7) with an IC50 of 13 nM. AY 9944 causes hypocholesterolaemia and accumulation of 7DHC. At high doses, AY 9944 also inhibits the sterol Δ7-Δ8 isomerase in cultured embryos, leading to the accumulation of cholesterol-8-en-3β-ol[1][2][3].AY 9944 affects the type of free sterols in keratinocytes at a concentration of 1 µg/ml for 15 h. AY 9944 inhibits the synthesis of cholesterol and desmosterol, but increases the synthesis of 7-dehydrocholesterol and oleanol[2].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.15mL

0.43mL

0.22mL

10.77mL

2.15mL

1.08mL

21.54mL

4.31mL

2.15mL
参考文献

[1]Moebius FF, et al. Molecular cloning and expression of the human delta7-sterol reductase. Proc Natl Acad Sci U S A. 1998 Feb 17;95(4):1899-902.

[2]Nemanic MK, et al. In vitro synthesis of vitamin D-3 by cultured human keratinocytes and fibroblasts: action spectrum and effect of AY-9944. Biochim Biophys Acta. 1985 Sep 6;841(3):267-77.

[3]Cortez MA, et al. Brain sterols in the AY-9944 rat model of atypical absence seizures. Epilepsia. 2002 Jan;43(1):3-8.