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WWL70

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Chemical Structure| 947669-91-2 同义名 : -
CAS号 : 947669-91-2
货号 : A739931
分子式 : C27H23N3O3
纯度 : 99%+
分子量 : 437.49
MDL号 : MFCD10567112
存储条件：

粉末 Sealed in dry,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 16 mg/mL(36.57 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 WWL70 is identified as a selective inhibitor of the alpha/beta hydrolase domain 6 (ABHD6), exhibiting an IC50 of 70 nM[1]. One hour following treatment with WWL70 at a concentration of 10 μM, there is a 20% increase in 2-Arachidonoylglycerol levels compared to untreated cells. WWL70, at concentrations of 1 μM or 10 μM, entirely prevents LPS-induced elevation of PGE2. It also diminishes the LPS-stimulated mRNA upregulation of mPGES-1 and mPGES-2. The IC50 for WWL70 in the inhibition of PGE2 synthesis is approximately 100 nM[2].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.29mL

0.46mL

0.23mL

11.43mL

2.29mL

1.14mL

22.86mL

4.57mL

2.29mL
参考文献

[1]Li W, Blankman JL, Cravatt BF. A functional proteomic strategy to discover inhibitors for uncharacterized hydrolases. J Am Chem Soc. 2007 Aug 8;129(31):9594-5. doi: 10.1021/ja073650c. Epub 2007 Jul 13. PMID: 17629278.

[2]Tanaka M, et al. WWL70 attenuates PGE2 production derived from 2-arachidonoylglycerol in microglia by ABHD6-independent mechanism. J Neuroinflammation. 2017 Jan 10;14(1):7.

[3]Tchantchou F, et al. Selective inhibition of alpha/beta-hydrolase domain 6 attenuates neurodegeneration, alleviates blood brain barrier breakdown, and improves functional recovery in a mouse model of traumatic brain injury. J Neurotrauma. 2013 Apr 1;30(7)