生物活性 | |||
---|---|---|---|
描述 | IRE1 (inositol requiring enzyme 1) is an ER transmembrane sensor that activates UPR to maintain ER and cellular function. It can promotes cell survive and initiate apoptosis via decay of anti-apoptotic microRNAs[2]. APY29 is a modulator of IRE1α by binding to the ATP-binding site and inhibits it autophosphorylation (IC50=280nM) and enhances its RNase function (EC50=460nM). In vitro, it restores the ability of dephosphoylated IRE1α to cleave XBP1 mRNA in a dose-dependent manner. It also enhances IRE1α oligomerization in a crosslinking assay[3]. |
实验方案 | |||
---|---|---|---|
1mg | 5mg | 10mg | |
1 mM 5 mM 10 mM |
3.01mL 0.60mL 0.30mL |
15.04mL 3.01mL 1.50mL |
30.09mL 6.02mL 3.01mL |
参考文献 |
---|