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NSC232003

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Chemical Structure| 1905453-18-0 同义名 : -
CAS号 : 1905453-18-0
货号 : A724205
分子式 : C6H7N3O3
纯度 : 99%+
分子量 : 169.138
MDL号 : MFCD31560476
存储条件：

粉末 Sealed in dry,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

H2O: 2 mg/mL(11.82 mM)，配合低频超声助溶
动物实验配方：
生物活性
靶点

  • E3 Ligase
描述 NSC232003 is a uracil derivative that can be used as a versatile lead compound for the development of UHRF1 inhibitors that can be used to analyse DNA methylation genetically.NSC232003 can be used as a potent DNA methylation inhibitor. NSC232003 was predicted to partially deprotonate the more acidic imide nitrogen on the pyrimidine ring at pH 7.6. After incubating U251 glioma cells with NSC232003 for 4 h, the interaction of DNMT1/UHRF1 was significantly attenuated, with 50% inhibition of the interaction at 15 μM, and DNMT1/UHRF1 induced global DNA cytosine deprotonation, as determined by ELISA [1].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

5.91mL

1.18mL

0.59mL

29.56mL

5.91mL

2.96mL

59.12mL

11.82mL

5.91mL
参考文献

[1]Myrianthopoulos V, et al. Tandem virtual screening targeting the SRA domain of UHRF1 identifies a novel chemical tool modulating DNA methylation. Eur J Med Chem. 2016 May 23;114:390-6.