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Aristolochic acid A

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Chemical Structure| 313-67-7 同义名 : 马兜铃酸 ;Aristolochic acid I;TR 1736;AristA;Birthwort;Tardolyt;NSC 50413;NSC 11926;Aristolochic Acid;Aristolochine;Aristolochin
CAS号 : 313-67-7
货号 : A714436
分子式 : C17H11NO7
纯度 : 99%+
分子量 : 341.272
MDL号 : MFCD00004996
存储条件：

粉末 Inert atmosphere,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 16 mg/mL(46.88 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 Aristolochic Acid A (Aristolochic acid I), is the main component of plant extract Aristolochic acids, which are found in various herbal plants of genus Aristolochia and Asarum, strongly induces toxic damage during ovarian maturation by inhibiting Akt phosphorylation-mediated suppression of apoptosis. Aristolochic acid A (150, 200 μM, 24 hours) inhibits the cell viabilities of kidney cells HEK293 and HK-2. Aristolochic acid A (100, 200 μM, 24 hours) causes a concentration-dependent decrease in bladder cancer-associated protein (BLCAP) mRNA levels in kidney cells (HEK 293 and HK-2), and bladder cancer cell line (HT-1376). Aristolochic acid A (100, 200 μM, 24 hours) weakens the BLCAP protein signals in a dose-dependent manner in both HEK293 and HT-1376 cells[3]. AA (Aristolochic Acid A) has been found to damage broilers' kidneys by breaking the redox balance to form oxidative stress, along with promoting apoptosis of renal cells[4]. Additionally, AAI promoted apoptosis in SSCs, which was accompanied by upregulation of caspase 3, P53 and BAX expression and downregulation of Bcl-2 expression, and suppressed autophagy, which was accompanied by upregulation of P62 expression and downregulation of ATG5 and LC3B expression, in a concentration-dependent manner. AAI (Aristolochic acid I) impaired spermatogenesis in rats, as identified by degeneration of the seminiferous epithelium, and increased apoptosis of testicular cells[5]. The mechanism of AA-I-induced hepatotoxicity was associated with oxidative-stress-mediated apoptosis and mitochondrial damage[6].
临床研究
NCT号 适应症或疾病 临床期 招募状态 预计完成时间 地点
NCT03066921 End Stage Renal Disease Not Applicable Completed - Taiwan ... 展开 >> Tungs' Taichung MetroHarbour Hospital Taichung, Taiwan 收起 <<
NCT01503645 - Unknown - Taiwan ... 展开 >> Far Estern Memorial Hospital Not yet recruiting Pan-Chiao, Taipei, Taiwan, 22060 National Taiwan University Hospital Recruiting Taipei, Taiwan, 10051 Contact: Kwan-Dun Wu, MD, PhD    +886-2-23123456 ext 5014    kdw@ntumc.org    Principal Investigator: Fe-Lin L Wu, Ph.D. 收起 <<
NCT00867633 - Unknown March 2012 Taiwan ... 展开 >> National Taiwan University Hospital Recruiting Taipei, Taiwan, 100 Contact: Yeong-Shiau Pu, Ph.D.    886-2-23123456 ext 65249    yspu@ntu.edu.tw 收起 <<
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.93mL

0.59mL

0.29mL

14.65mL

2.93mL

1.47mL

29.30mL

5.86mL

2.93mL
参考文献

[1]Kwak DH, Park JH, et al. Aristolochic Acid I induces ovarian toxicity by inhibition of akt phosphorylation. Chem Res Toxicol. 2014 Dec 15;27(12):2128-35.

[2]Pan JH, Yan GJ, Song J. [The determination of aristolochic acid A in different processed Aristolochia manshuriensis and the test of influence about renal function in rats] . Zhong Yao Cai. 2010 Aug;33(8):1228-33. Chinese.

[3]Huang YT, Wu TS, Lu CC, Yu FY, Liu BH. Aristolochic acid I interferes with the expression of BLCAP tumor suppressor gene in human cells. Toxicol Lett. 2018 Jul;291:129-137

[4]Xu D, Ran C, Yin L, Lin J, Fu H, Peng X, Zhao X, Shu G. Acute and Subchronic Toxicity Studies of Aristolochic Acid A in Tianfu Broilers. Animals (Basel). 2021 May 27;11(6):1556

[5]Liu Y, He X, Wang Y, Zhou H, Zhang Y, Ma J, Wang Z, Yang F, Lu H, Yang Y, Deng Z, Qi X, Gong L, Ren J. Aristolochic acid I induces impairment in spermatogonial stem cell in rodents. Toxicol Res (Camb). 2021 Apr 26;10(3):436-445

[6]Xu D, Yin L, Lin J, Fu H, Peng X, Chang L, Zheng Y, Zhao X, Shu G. Aristolochic Acid I-Induced Hepatotoxicity in Tianfu Broilers Is Associated with Oxidative-Stress-Mediated Apoptosis and Mitochondrial Damage. Animals (Basel). 2021 Dec 2;11(12):3437