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TTNPB (Arotinoid Acid)

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Chemical Structure| 71441-28-6 同义名 : Ro 13-7410;Arotinoid acid;AGN191183;TTNPB
CAS号 : 71441-28-6
货号 : A700949
分子式 : C24H28O2
纯度 : 99%+
分子量 : 348.478
MDL号 : MFCD00673917
存储条件：

粉末 Sealed in dry,Store in freezer, under -20°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 7 mg/mL(20.09 mM)，配合低频超声，并水浴加热至45℃助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 TTNPB (Ro 13-7410) (GMP) refers to TTNPB produced following GMP guidelines. GMP small molecules serve as appropriate auxiliary reagents for cell therapy manufacture. TTNPB is a highly potent retinoic acid receptor (RAR) agonist.
细胞研究
细胞系 浓度 检测类型 检测时间 活动说明 数据源
CV-1 cells Function assay Binding affinity against retinoic Acid gamma receptors co-transfected into CV-1 cells, EC50=0.002 Μm 8308867
HEK293 cells Function assay Agonist activity at human RARalpha expressed in HEK293 cells by luciferase reporter gene assay, EC50=0.18 nM 25305688
HeLa cells Function assay Agonist activity at human RARalpha expressed in human HeLa cells assessed as relative luminescence units at >=10 uM by luciferase assay relative to control 18951029
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.87mL

0.57mL

0.29mL

14.35mL

2.87mL

1.43mL

28.70mL

5.74mL

2.87mL
参考文献

[1]Pignatello MA, et al. Multiple factors contribute to the toxicity of the aromatic retinoid, TTNPB (Ro 13-7410): binding affinities and disposition. Toxicol Appl Pharmacol. 1997 Feb;142(2):319-27.

[2]Manabu Kawata, et al. Simple and Robust Differentiation of Human Pluripotent Stem Cells toward Chondrocytes by Two Small-Molecule Compounds. Stem Cell Reports. 2019 Sep 10;13(3):530-544.