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MMAF-OMe

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Chemical Structure| 863971-12-4 同义名 : Monomethyl auristatin F methyl ester
CAS号 : 863971-12-4
货号 : A700623
分子式 : C40H67N5O8
纯度 : 99%+
分子量 : 745.989
MDL号 : MFCD30535529
存储条件：

粉末 Keep in dark place,Inert atmosphere,Store in freezer, under -20°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 105 mg/mL(140.75 mM)，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 MMAF-Ome is a methoxyl analogue of MMAF, used in antibody-drug conjugates (ADCs) as the antimitotic agent part[3]. The methoxyl works as the reaction group. MMAF is a potent auristatin-derived antineoplastic agent, working by inhibition of tubulin polymerization[4]. It exhibited cytotoxicity in Karpas 299, H3396, 786-O and Caki-1 cells with IC50 values of 119nM, 105nM, 257nM and 200nM after 96-hour treatment[3].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

1.34mL

0.27mL

0.13mL

6.70mL

1.34mL

0.67mL

13.41mL

2.68mL

1.34mL
参考文献

[1]Doronina SO, Mendelsohn BA, et al. Enhanced activity of monomethylauristatin F through monoclonal antibody delivery: effects of linker technology on efficacy and toxicity. Bioconjug Chem. 2006 Jan-Feb;17(1):114-24.

[2]Chen H, Lin Z, et al. Tubulin Inhibitor-Based Antibody-Drug Conjugates for Cancer Therapy. Molecules. 2017 Aug 1;22(8). pii: E1281.

[3]Doronina SO, Mendelsohn BA, Bovee TD, Cerveny CG, Alley SC, Meyer DL, Oflazoglu E, Toki BE, Sanderson RJ, Zabinski RF, Wahl AF, Senter PD. Enhanced activity of monomethylauristatin F through monoclonal antibody delivery: effects of linker technology on efficacy and toxicity. Bioconjug Chem. 2006 Jan-Feb;17(1):114-24. doi: 10.1021/bc0502917. PMID: 16417259.

[4]Chen H, Lin Z, Arnst KE, Miller DD, Li W. Tubulin Inhibitor-Based Antibody-Drug Conjugates for Cancer Therapy. Molecules. 2017 Aug 1;22(8):1281. doi: 10.3390/molecules22081281. PMID: 28763044; PMCID: PMC6152078.