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Ganoderic acid A

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Chemical Structure| 81907-62-2 同义名 : -
CAS号 : 81907-62-2
货号 : A684127
分子式 : C30H44O7
纯度 : 95%
分子量 : 516.666
MDL号 : MFCD26131275
存储条件：

粉末 Sealed in dry,Store in freezer, under -20°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 60 mg/mL(116.13 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO

无水乙醇: 50 mg/mL(96.77 mM)，注意：无水乙醇开封后，易挥发，也会吸收空气中的水分，导致溶解能力下降，请避免使用开封较久的乙醇
动物实验配方：
生物活性
描述 Ganoderic acid A (GAA), a representative active triterpenoid from Ganoderma lucidum, has been reported to exhibit antinociceptive, antioxidative, cytotoxic, hepatoprotective and anticancer activities[3]. GA-A (6.25-100 μM) significantly inhibited cyst growth in MDCK cyst model and embryonic kidney cyst model in vitro, and the inhibitory effect was reversible. In kidney-specific Pkd1 knockout (kPKD) mice displaying severe cystic kidney disease, administration of GA-A (50 mg· kg-1 ·d-1, sc) significantly attenuated renal cyst development[4]. Pretreatment with GA A (10 μM) significantly attenuated SNP(sodium nitroprusside)-induced cytotoxicity and NO (nitric oxide) increase in SH-SY5Y cells, but not in PC12 cells. Furthermore, pretreatment with GA A caused significantly higher adrenaline content in SH-SY5Y cells than in PC12 cells[5]. GAA attenuates acute lung injury in mouse model via the inhibition of Rho/ROCK/NF-κB pathway[6].
细胞研究
细胞系 浓度 检测类型 检测时间 活动说明 数据源
Bel7402 cells Cytotoxicity assay Cytotoxicity against human Bel7402 cells, IC50=7.25 μM 23092389
HepG2 cells Function assay 48 h Anticancer activity against human HepG2 cells assessed as cell viability after 48 hrs by MTT assay, IC50=38.7 μM 24974349
P388 cells Cytotoxicity assay Cytotoxicity against mouse P388 cells, IC50=7.25 μM 23092389
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

1.94mL

0.39mL

0.19mL

9.68mL

1.94mL

0.97mL

19.35mL

3.87mL

1.94mL
参考文献

[1]Radwan FF, Hossain A, et al. Reduction of myeloid-derived suppressor cells and lymphoma growth by a natural triterpenoid. J Cell Biochem. 2015 Jan;116(1):102-14.

[2]Yao X, Li G, et al. Inhibition of the JAK-STAT3 signaling pathway by ganoderic acid A enhances chemosensitivity of HepG2 cells to cisplatin. Planta Med. 2012 Nov;78(16):1740-8.

[3]Cao FR, Feng L, Ye LH, Wang LS, Xiao BX, Tao X, Chang Q. Ganoderic Acid A Metabolites and Their Metabolic Kinetics. Front Pharmacol. 2017 Mar 7;8:101

[4]Meng J, Sai-Zhen Wang, He JZ, Zhu S, Huang BY, Wang SY, Li M, Zhou H, Lin SQ, Yang BX. Ganoderic acid A is the effective ingredient of Ganoderma triterpenes in retarding renal cyst development in polycystic kidney disease. Acta Pharmacol Sin. 2020 Jun;41(6):782-790

[5]Yu ZR, Jia WH, Liu C, Wang HQ, Yang HG, He GR, Chen RY, Du GH. Ganoderic acid A protects neural cells against NO stress injury in vitro via stimulating β adrenergic receptors. Acta Pharmacol Sin. 2020 Apr;41(4):516-522

[6]Wan B, Li Y, Sun S, Yang Y, Lv Y, Wang L, Song M, Chen M, Wu C, Pan H, Zhang X. Ganoderic acid A attenuates lipopolysaccharide-induced lung injury in mice. Biosci Rep. 2019 May 23;39(5):BSR20190301