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SIBA

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Chemical Structure| 35899-54-8 同义名 : 5'-Deoxy-5'-isobutylthioadenosine;5'-Isobutylthioadenosine
CAS号 : 35899-54-8
货号 : A654709
分子式 : C14H21N5O3S
纯度 : 99%+
分子量 : 339.413
MDL号 : MFCD00056008
存储条件：

粉末 Keep in dark place,Inert atmosphere,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 105 mg/mL(309.36 mM)，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 Muscarinic receptors have been tentatively subclassified to M1 and M2 on the basis of differences in their affinity for the cholinergic antagonist pirenzepine. 5'-isobutylthio-adenosine (SIBA) is best known as inhibitor of S-adenosylhomocysteine hydrolase, but has also been implicated as inhihitors of protein synthesis, nucleic acid synthesis, viral replication, cellular differentiation, chemotaxis, polyamine metabolism and IgE-mediated histamine release. Most of these effects have been attributed to the inhibition of transmethylation reactions. SIBA also has biological and biochemical effects over muscarinic receptors other than on transmethylation reactions. SIBA, at 100 µM, inhibited [3H]N-methyl-scopolamine binding to homogenates of whole brain and cortex (mainly M1 subtype receptors) by 11-30% and to cerebellum (mainly M2 subtype receptors) by 20-39%. At 0.01-1.0 µM, stimulation of [3H]QNB and NMS-inaccessible [3H]QNB binding was observed, with the most induced by 1 µM 3-deaza-SIBA. In contrast, [3H]pirenzepine ([3H]PZ) binding to whole brain and cortex was inhibited in a dose-dependent manner with Ki values in the µM range[3].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.95mL

0.59mL

0.29mL

14.73mL

2.95mL

1.47mL

29.46mL

5.89mL

2.95mL
参考文献

[1]Lawrence F, Bachrach U, et al. Effect of 5'-deoxy-5'-isobutylthioadenosine on putrescine uptake and polyamine biosynthesis by chick embryo fibroblasts. Biochem J. 1982 Jun 15;204(3):853-9.

[2]Carteni-Farina M, Della Ragione F, et al. Studies on the metabolism of 5'-isobutylthioadenosine (SIBA): phosphorolytic cleavage by methylthioadenosine phosphorylase. FEBS Lett. 1979 Aug 15;104(2):266-70.

[3]Muscarinic Receptor Subtype Specificity of 5'-(isobutylthio)-adenosine (SIBA) and Its Analogs