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PBIT

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Chemical Structure| 2514-30-9 同义名 : 2-​p-​tolyl-1,​2-​Benzisothiazolin-​3-​one
CAS号 : 2514-30-9
货号 : A626958
分子式 : C14H11NOS
纯度 : 95%
分子量 : 241.308
MDL号 : MFCD05856231
存储条件：

粉末 Sealed in dry,Room Temperature

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 50 mg/mL(207.2 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 PBIT is a specific inhibitor of JARID1. It inhibits the histone demethylase activity of JARID1B (also known as KDM5B or PLU1), with an IC50 of approximately 3 μM. PBIT also inhibits JARID1A and JARID1C, with IC50 values of 6 μM and 4.9 μM, respectively. PBIT suppresses the proliferation of cells expressing high levels of JARID1B. The inhibition of cell proliferation by PBIT is dependent on the level of JARID1B, specifically at concentrations of 1-10 μM in UACC-812 cells and 2.5-10 μM in MCF7 and MCF10A cells over a period of 72 hours[1].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

4.14mL

0.83mL

0.41mL

20.72mL

4.14mL

2.07mL

41.44mL

8.29mL

4.14mL
参考文献

[1]Sayegh J, et al. Identification of small molecule inhibitors of Jumonji AT-rich interactive domain 1B (JARID1B) histone demethylase by a sensitive high throughput screen. J Biol Chem. 2013 Mar 29;288(13):9408-17.