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DNQX

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Chemical Structure| 2379-57-9 同义名 : FG 9041;6,7-Dinitroquinoxaline-2,3-dione
CAS号 : 2379-57-9
货号 : A622616
分子式 : C8H4N4O6
纯度 : 99%+
分子量 : 252.141
MDL号 : MFCD00069257
存储条件：

粉末 Sealed in dry,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 35 mg/mL(138.81 mM)，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 DNQX, a specific AMPA receptor antagonist, given as either a 5 mg/kg or 10 mg/kg intraperitoneal dose or into the lateral cerebral ventricle (5 microliters of 0.5 mg/ml) significantly diminished PCP (40 mg/kg) and ketamine (80, 100, 120 mg/kg) hsp70 induction in the posterior cingulate and retrosplenial cortex. The most dramatic decrease of hsp70 induction was seen with the intraventricular dose of DNQX[3]. Bilateral injection of DNQX (1 microgram/0.5 microliters/side) inhibited acquisition of place preference to amphetamine (1 mg/kg) but not morphine (10 mg/kg). During acquisition, DNQX marginally attenuated the locomotor stimulation elicited by amphetamine during the first but not subsequent training sessions, while the combination of morphine plus DNQX produced marked akinesia during each training session[4]. DNQX (6,7-dinitroquinoxaline-2,3-dione), as a quinoxaline derivatives, is a non-NMDA (N-methyl-D-aspartate) receptor antagonist. DNQX and CNQX (6-cyano-7-nitroquinoxaline-2,3-dione) produced a consistent depolarization in all TRN (thalamic reticular nucleus) neurons tested. The DNQX- and CNQX-mediated depolarizations are mediated by AMPAR (transmembrane AMPA receptor) but not kainate receptors in TRN neurons. Quinoxaline derivatives could modulate synaptic transmission and alter neuronal excitability[5]. DNQX toxic effect is neuron-specific since cultured hippocampal glial cells are unaffected. DNQX toxicity in cultured hippocampal neurons is apparently mediated through an ionotropic glutamate receptor-independent way[6].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

3.97mL

0.79mL

0.40mL

19.83mL

3.97mL

1.98mL

39.66mL

7.93mL

3.97mL
参考文献

[1]Watkins JC, Krogsgaard-Larsen P, et al. Structure-activity relationships in the development of excitatory amino acid receptor agonists and competitive antagonists. Trends Pharmacol Sci. 1990 Jan;11(1):25-33.

[2]Honore T, Davies SN, et al. Quinoxalinediones: potent competitive non-NMDA glutamate receptor antagonists. Science. 1988 Aug 5;241(4866):701-3.

[3]Sharp JW, Petersen DL, Langford MT. DNQX inhibits phencyclidine (PCP) and ketamine induction of the hsp70 heat shock gene in the rat cingulate and retrosplenial cortex. Brain Res. 1995;687(1-2):114-124

[4]Layer RT, Uretsky NJ, Wallace LJ. Effects of the AMPA/kainate receptor antagonist DNQX in the nucleus accumbens on drug-induced conditioned place preference. Brain Res. 1993;617(2):267-273

[5]Lee SH, Govindaiah G, Cox CL. Selective excitatory actions of DNQX and CNQX in rat thalamic neurons. J Neurophysiol. 2010;103(4):1728-1734

[6]Martin A, Récasens M, Guiramand J. DNQX-induced toxicity in cultured rat hippocampal neurons: an apparent AMPA receptor-independent effect?. Neurochem Int. 2003;42(3):251-260