产品说明书

WS3

Print
Chemical Structure| 1421227-52-2 同义名 : -
CAS号 : 1421227-52-2
货号 : A606355
分子式 : C28H30F3N7O3
纯度 : 99%+
分子量 : 569.578
MDL号 : MFCD26142950
存储条件：

粉末 Sealed in dry,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 2 mg/mL(3.51 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 WS3 is a novel proliferative molecule that promotes pancreatic β cell proliferation in rodent and human primary islets. WS3 can be used for the research of type 1 diabetes. WS3 induces pancreatic R7T1 β cell proliferation in dose response, with an EC50 value of 0.28 μM[3]. WS3 (1.0 nM-1.0 µM) reversibly proliferate primary retinal pigment epithelial (RPE) cells isolated from fetal and adult human donors. Following withdrawal of WS3, RPE cells differentiate into a functional monolayer, as exhibited by their expression of mature RPE genes and phagocytosis of photoreceptor outer segments[4]. WS1 and WS3 are caused by mutation in the gene encoding the transcription factor Pax3, which regulates MITF (Microphthalmia-associated transcription factor) expression[5].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

1.76mL

0.35mL

0.18mL

8.78mL

1.76mL

0.88mL

17.56mL

3.51mL

1.76mL
参考文献

[1]Shen W, Tremblay MS, et al. Small-molecule inducer of β cell proliferation identified by high-throughput screening. J Am Chem Soc. 2013 Feb 6;135(5):1669-72.

[2]Swoboda JG, Elliott J, et al. Small molecule mediated proliferation of primary retinal pigment epithelial cells. ACS Chem Biol. 2013 Jul 19;8(7):1407-11.

[3]Shen W, Tremblay MS, Deshmukh VA, Wang W, Filippi CM, Harb G, Zhang YQ, Kamireddy A, Baaten JE, Jin Q, Wu T, Swoboda JG, Cho CY, Li J, Laffitte BA, McNamara P, Glynne R, Wu X, Herman AE, Schultz PG. Small-molecule inducer of β cell proliferation identified by high-throughput screening. J Am Chem Soc. 2013 Feb 6;135(5):1669-72

[4]Swoboda JG, Elliott J, Deshmukh V, de Lichtervelde L, Shen W, Tremblay MS, Peters EC, Cho CY, Lu B, Girman S, Wang S, Schultz PG. Small molecule mediated proliferation of primary retinal pigment epithelial cells. ACS Chem Biol. 2013 Jul 19;8(7):1407-11

[5]Verastegui C, Bille K, Ortonne JP, Ballotti R. Regulation of the microphthalmia-associated transcription factor gene by the Waardenburg syndrome type 4 gene, SOX10. J Biol Chem. 2000 Oct 6;275(40):30757-60