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Daidzin

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Chemical Structure| 552-66-9 同义名 : 黄豆苷 ;Daidzoside;NPI-031D;Daidzein 7-glucoside;Daidzein 7-O-glucoside
CAS号 : 552-66-9
货号 : A590254
分子式 : C21H20O9
纯度 : 98+%
分子量 : 416.378
MDL号 : -
存储条件：

粉末 Inert atmosphere,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 250 mg/mL(600.42 mM)，配合低频超声，并水浴加热至45℃助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
靶点

  • Dehydrogenase

    ALDH-Ⅰ, Ki:20 nM
描述 Daidzin is also a potent and selective inhibitor of human mitochondrial aldehyde dehydrogenase (ALDH-2) with IC50 of 80 nM[3]. Daidzin is also an isoflavone that has anti-oxidant, anti-carcinogenic, and anti-atherosclerotic activities[4]. Daidzin possess tyrosyl radical scavenging activity and thereby decreases the oxidative stress. Treatment with daidzin protects the cornea in dry eye rat model by suppression inflammation and oxidative stress[5]. ITG extract and its active component Daidzin effectively improved CYP-induced oxidative stress, inflammation, and fibrosis through inhibiting the MMP-8, TIMP-1, and oxidative stress[6]. Daidzin treatment (150 mg/kg per day i.p. for 6 days) significantly suppresses (> 70%) hamster ethanol intake but does not affect overall acetaldehyde metabolism. In vitro, daidzin suppresses hamster liver mitochondria-catalyzed acetaldehyde oxidation very potently with an IC50 value of 0.4 mM, which is substantially lower than the daidzin concentration (70 mM) found in the liver mitochondria of daidzin-treated hamsters[7]. Daidzin inhibits ALDH-I competitively with respect to formaldehyde with a Ki of 40 nM, and uncompetitively with respect to the coenzyme NAD+[8].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.40mL

0.48mL

0.24mL

12.01mL

2.40mL

1.20mL

24.02mL

4.80mL

2.40mL
参考文献

[1]Keung WM, Vallee BL. Kudzu root: an ancient Chinese source of modern antidipsotropic agents. Phytochemistry. 1998 Feb;47(4):499-506.

[2]Keung WM, Vallee BL. Daidzin: a potent, selective inhibitor of human mitochondrial aldehyde dehydrogenase. Proc Natl Acad Sci U S A. 1993 Feb 15;90(4):1247-51.

[3]Keung WM, Klyosov AA, Vallee BL. Daidzin inhibits mitochondrial aldehyde dehydrogenase and suppresses ethanol intake of Syrian golden hamsters. Proc Natl Acad Sci U S A. 1997 Mar 4;94(5):1675-9

[4]Xie CI, Lin RC, Antony V, Lumeng L, Li TK, Mai K, Liu C, Wang QD, Zhao ZH, Wang GF. Daidzin, an antioxidant isoflavonoid, decreases blood alcohol levels and shortens sleep time induced by ethanol intoxication. Alcohol Clin Exp Res. 1994 Dec;18(6):1443-7

[5]Xiao F, Cui H, Zhong X. Beneficial effect of daidzin in dry eye rat model through the suppression of inflammation and oxidative stress in the cornea. Saudi J Biol Sci. 2018 May;25(4):832-837

[6]Wu KC, Lin WY, Sung YT, Wu WY, Cheng YH, Chen TS, Chiang BJ, Chien CT. Glycine tomentella hayata extract and its ingredient daidzin ameliorate cyclophosphamide-induced hemorrhagic cystitis and oxidative stress through the action of antioxidation, anti-fibrosis, and anti-inflammation. Chin J Physiol. 2019 Sep-Oct;62(5):188-195

[7]Keung WM, Lazo O, Kunze L, Vallee BL. Daidzin suppresses ethanol consumption by Syrian golden hamsters without blocking acetaldehyde metabolism. Proc Natl Acad Sci U S A. 1995 Sep 12;92(19):8990-3

[8]Keung WM, Vallee BL. Daidzin: a potent, selective inhibitor of human mitochondrial aldehyde dehydrogenase. Proc Natl Acad Sci U S A. 1993 Feb 15;90(4):1247-51