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Doxycycline

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Chemical Structure| 564-25-0 同义名 : 强力霉素 ;Vibramycin;Doxiciclina;Doxycyclinum;Doxytetracycline
CAS号 : 564-25-0
货号 : A587145
分子式 : C22H24N2O8
纯度 : 98%
分子量 : 444.435
MDL号 : MFCD00800994
存储条件：

粉末 Inert atmosphere,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 105 mg/mL(236.26 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
靶点

  • MMP
描述 Doxycycline, an antibiotic, is an orally active and broad-spectrum metalloproteinase (MMP) inhibitor[1]. Doxycycline doses commonly used with inducible expression systems (0.01-1 µg/mL) substantially alter cellular metabolism: mitochondrial protein synthesis was inhibited accompanied by reduced oxygen and increased glucose consumption. Furthermore, doxycycline protected human glioma cells from hypoxia-induced cell death. An impairment of cell growth was only detectable with higher doxycycline doses (10 µg/mL)[2]. Doxycycline had a deleterious effect on control groups and positive action for bone organization on female rats affected by bilateral ovariectomy-induced osteopenia and sedentary lifestyle[3]. The treatment with doxycycline and tocopherol alone and in combination significantly attenuated locomotor activity, memory recognition, reduced neuroinflammation, preserved oxidative balance, and restored the level of neurotransmitters[4]. Doxycycline caused certain fetal abnormalities, so it is advisable to avoid using this drug during pregnancy[5].
临床研究
NCT号 适应症或疾病 临床期 招募状态 预计完成时间 地点
NCT00177333 Abortion, Induced ... 展开 >> Vomiting 收起 << Phase 4 Completed - United States, Pennsylvania ... 展开 >> Magee-Womens Hospital Pittsburgh, Pennsylvania, United States, 15213 收起 <<
NCT02623959 Advanced Cancers ... 展开 >> Malignant Pleural Effusions 收起 << Not Applicable Terminated(Slow Accrual) - United States, Texas ... 展开 >> University of Texas MD Anderson Cancer Center Houston, Texas, United States, 77030 收起 <<
NCT03115177 Osteoarthritis Not Applicable Completed - United States, Illinois ... 展开 >> Rush University Medical Center Chicago, Illinois, United States, 60612 收起 <<
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.25mL

0.45mL

0.23mL

11.25mL

2.25mL

1.13mL

22.50mL

4.50mL

2.25mL
参考文献

[1]Briest W, Cooper TK, Tae HJ, Krawczyk M, McDonnell NB, Talan MI. Doxycycline ameliorates the susceptibility to aortic lesions in a mouse model for the vascular type of Ehlers-Danlos syndrome. J Pharmacol Exp Ther. 2011 Jun;337(3):621-7

[2]Luger AL, Sauer B, Lorenz NI, Engel AL, Braun Y, Voss M, Harter PN, Steinbach JP, Ronellenfitsch MW. Doxycycline Impairs Mitochondrial Function and Protects Human Glioma Cells from Hypoxia-Induced Cell Death: Implications of Using Tet-Inducible Systems. Int J Mol Sci. 2018 May 17;19(5):1504

[3]de Figueiredo FAT, Shimano RC, Ervolino E, Pitol DL, Gerlach RF, Issa JPM. Doxycycline reduces osteopenia in female rats. Sci Rep. 2019 Oct 25;9(1):15316

[4]Rana A, Singh S, Deshmukh R, Kumar A. Pharmacological potential of tocopherol and doxycycline against traumatic brain injury-induced cognitive/motor impairment in rats. Brain Inj. 2020 Jul 2;34(8):1039-1050

[5]Abd-Allah ER, Abd El-Rahman HA. Influence of doxycycline administration on rat embryonic development during organogenesis. J Biochem Mol Toxicol. 2021 Jan;35(1):e2261