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Isoscopoletin

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Chemical Structure| 776-86-3 同义名 : 6-Hydroxy-7-methoxycoumarin;Esculetin 7-methyl ether;7-methoxy-6-Hydroxycoumarin;7-Methoxyesculetin
CAS号 : 776-86-3
货号 : A554035
分子式 : C10H8O4
纯度 : 99%+
分子量 : 192.168
MDL号 : MFCD00016976
存储条件：

粉末 Sealed in dry,Room Temperature

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 105 mg/mL(546.4 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 Isoscopoletin (6-Hydroxy-7-methoxycoumarin) is an active constituent in Artemisia argyi leaves. Isoscopoletin shows substantial inhibition against cell proliferation, with IC50s of 4.0 μM and 1.6 μM for human CCRF-CEM leukaemia cells and multidrug resistant subline CEM/ADR5000, respectively[3]. Isoscopoletin possesses inhibitory activity against HBV (hepatitis B virus) replication[4]. Isoscopoletin showed significantly cytotoxic effects against three cell lines (A549, MCF7 and HepG2), 1 and 5 against two cell lines (A549 and MCF7), and 6 against one cell line (MCF7). Its IC50 values ranged between 2.73 ± 0.86 μM and 24.14 ± 3.12 μM[5].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

5.20mL

1.04mL

0.52mL

26.02mL

5.20mL

2.60mL

52.04mL

10.41mL

5.20mL
参考文献

[1]Cheng F, Deng Z, et al. Machilusmarin, a new neuroprotective isocoumarin dimer from the stems of Machilus ichangensis Rehd. et Wils. Nat Prod Res. 2013;27(17):1542-7.

[2]Adams M, Efferth T, Bauer R. Activity-guided isolation of scopoletin and isoscopoletin, the inhibitory active principles towards CCRF-CEM leukaemia cells and multi-drug resistant CEM/ADR5000 cells, from Artemisia argyi. Planta Med. 2006 Jul;72(9):862-4.

[3]Adams M, Efferth T, Bauer R. Activity-guided isolation of scopoletin and isoscopoletin, the inhibitory active principles towards CCRF-CEM leukaemia cells and multi-drug resistant CEM/ADR5000 cells, from Artemisia argyi. Planta Med. 2006 Jul;72(9):862-4

[4]Li H, Zhou C, Pan Y, Gao X, Wu X, Bai H, Zhou L, Chen Z, Zhang S, Shi S, Luo J, Xu J, Chen L, Zheng X, Zhao Y. Evaluation of antiviral activity of compounds isolated from Ranunculus sieboldii and Ranunculus sceleratus. Planta Med. 2005 Dec;71(12):1128-33

[5]Zhao DD, Zhao QS, Liu L, Chen ZQ, Zeng WM, Lei H, Zhang YL. Compounds from Dryopteris fragrans (L.) Schott with cytotoxic activity. Molecules. 2014 Mar 18;19(3):3345-55