产品说明书

Coelenterazine

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Chemical Structure| 55779-48-1 同义名 : Luciferin (Oplophorus);Preluciferin (Watasenia);Coelenterazine, Coelenterazine;CLZN;Oplophorus, luciferin
CAS号 : 55779-48-1
货号 : A544127
分子式 : C26H21N3O3
纯度 : 99%+
分子量 : 423.463
MDL号 : MFCD00467176
存储条件：

粉末 Keep in dark place,Inert atmosphere,Store in freezer, under -20°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

无水乙醇: 1 mg/mL(2.36 mM)，配合低频超声，并水浴加热至45℃助溶，注意：无水乙醇开封后，易挥发，也会吸收空气中的水分，导致溶解能力下降，请避免使用开封较久的乙醇
动物实验配方：
生物活性
描述 Coelenterazine is a luciferin found in many aquatic organisms across eight phyla. It is luminophore of the aequorin complex which is oxidized by oxygen to illuminate at 465nm. It is used for bioluminescence, such as measuring the intracellular calcium concentration[6], aequorin assays[7] and in vivo bioluminescence imaging[8]. Coelenterazine should be protected from exposure to light.
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.36mL

0.47mL

0.24mL

11.81mL

2.36mL

1.18mL

23.61mL

4.72mL

2.36mL
参考文献

[1]Pandey D, Fulton DJ, et al. Molecular regulation of NADPH oxidase 5 via the MAPK pathway. Am J Physiol Heart Circ Physiol. 2011 Apr;300(4):H1336-44.

[2]Ritsick DR, Edens WA, et al. Nox regulation of smooth muscle contraction. Free Radic Biol Med. 2007 Jul 1;43(1):31-8. Epub 2007 Mar 12.

[3]Gentschev I, Stritzker J, et al. Use of an oncolytic vaccinia virus for the treatment of canine breast cancer in nude mice: preclinical development of a therapeutic agent. Cancer Gene Ther. 2009 Apr;16(4):320-8.

[4]Bhaumik S, Gambhir SS, et al. Optical imaging of Renilla luciferase reporter gene expression in living mice. Proc Natl Acad Sci U S A. 2002 Jan 8;99(1):377-82. Epub 2001 Dec 18.

[5]Bronsart LL, Stokes C, et al. Chemiluminescence Imaging of Superoxide Anion Detects Beta-Cell Function and Mass. PLoS One. 2016 Jan 11;11(1):e0146601.

[6]Pandey D, Fulton DJ. Molecular regulation of NADPH oxidase 5 via the MAPK pathway. Am J Physiol Heart Circ Physiol. 2011 Apr;300(4):H1336-44. doi: 10.1152/ajpheart.01163.2010. Epub 2011 Feb 4. PMID: 21297032; PMCID: PMC3075021.

[7]Ritsick DR, Edens WA, Finnerty V, Lambeth JD. Nox regulation of smooth muscle contraction. Free Radic Biol Med. 2007 Jul 1;43(1):31-8. doi: 10.1016/j.freeradbiomed.2007.03.006. Epub 2007 Mar 12. PMID: 17561091; PMCID: PMC1989158.

[8]Gentschev I, Stritzker J, Hofmann E, Weibel S, Yu YA, Chen N, Zhang Q, Bullerdiek J, Nolte I, Szalay AA. Use of an oncolytic vaccinia virus for the treatment of canine breast cancer in nude mice: preclinical development of a therapeutic agent. Cancer Gene Ther. 2009 Apr;16(4):320-8. doi: 10.1038/cgt.2008.87. Epub 2008 Oct 24. PMID: 18949014.