产品说明书

Mitapivat

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Chemical Structure| 1260075-17-9 同义名 : AG-348
CAS号 : 1260075-17-9
货号 : A537823
分子式 : C24H26N4O3S
纯度 : 99%+
分子量 : 450.553
MDL号 : MFCD26936342
存储条件：

粉末 Sealed in dry,Store in freezer, under -20°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 20 mg/mL(44.39 mM)，配合低频超声，并水浴加热至45℃助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 Mitapivat (AG-348) functions as an orally active allosteric activator of pyruvate kinase. It enhances enzymatic activity, protein stability, and ATP levels across various PKLR genotypes, indicating its potential in restoring the function of PK (pyruvate kinase)-deficient glycolytic pathways. Mitapivat is applicable in the investigation of PK deficiency [1][2][3].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.22mL

0.44mL

0.22mL

11.10mL

2.22mL

1.11mL

22.19mL

4.44mL

2.22mL
参考文献

[1]Kung C, et al. AG-348 enhances pyruvate kinase activity in red blood cells from patients with pyruvate kinase deficiency. Blood. 2017 Sep 14;130(11):1347-1356.

[2]Matte A, et al. The pyruvate kinase activator mitapivat reduces hemolysis and improves anemia in a β-thalassemia mouse model. J Clin Invest. 2021 May 17;131(10):e144206.

[3]Rab MAE, et al. AG-348 (Mitapivat), an allosteric activator of red blood cell pyruvate kinase, increases enzymatic activity, protein stability, and ATP levels over a broad range of PKLR genotypes. Haematologica. 2021 Jan 1;106(1):238-249.