产品说明书

D-Sphingosine

Print
Chemical Structure| 123-78-4 同义名 : 鞘氨醇 ;Erythrosphingosine;trans-4-Sphingenine;Sphingosine (d18:1);D-erythro-Sphingosine C-18;(−)-Sphingosine;Sphingosine, C18 chain;(2S,3R)-D-erythro-2-amino-1,3-Octadec-4E-enediol;Sphingosine;erythro-C18-Sphingosine;D-erythro-Sphingosine
CAS号 : 123-78-4
货号 : A536742
分子式 : C18H37NO2
纯度 : 98%
分子量 : 299.492
MDL号 : MFCD00036751
存储条件：

粉末 Keep in dark place,Sealed in dry,Store in freezer, under -20°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 50 mg/mL(166.95 mM)，配合低频超声，并水浴加热至45℃助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 Protein kinase C, a family of related serine/threonine kinases implicated in multiple signal transduction pathways, exhibits antitumor property in mammalian cells. D-erythro-Sphingosine is a PKC inhibitor with an EC50 value of 8 ± 3 μM for p32. Treatment of Jurkat T cells with D-erythro-Sphingosine led to a ~4-fold increase in p32 phosphorylation, with the maximal effect observed at 10 μM[1]. D-erythro-Sphingosine is also a PP2A agonist that activates PP2A in neuronal cultures at a concentration of 10 nM. Rats treated with D-erythro-Sphingosine (1 μM) showed a higher locomotor BBB score after spinal cord injury compared to the vehicle-treated control group[2].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

3.34mL

0.67mL

0.33mL

16.69mL

3.34mL

1.67mL

33.39mL

6.68mL

3.34mL
参考文献

[1]Pushkareva MYu, Bielawska A, Menaldiv D, Liotta D, Hannun YA. Regulation of sphingosine-activated protein kinases: selectivity of activation by sphingoid bases and inhibition by non-esterified fatty acids. Biochem J. 1993 Sep 15;294 (Pt 3)(Pt 3):699-703. doi: 10.1042/bj2940699

[2]Cheng P, Chen K, Yu W, Gao S, Hu S, Sun X, Huang H. Protein phosphatase 2A (PP2A) activation promotes axonal growth and recovery in the CNS. J Neurol Sci. 2015 Dec 15;359(1-2):48-56. doi: 10.1016/j.jns.2015.10.025