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DMNB

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Chemical Structure| 20357-25-9 同义名 : 6-Nitroveratraldehyde;NSC 65590
CAS号 : 20357-25-9
货号 : A506283
分子式 : C9H9NO5
纯度 : 97%
分子量 : 211.172
MDL号 : MFCD00007134
存储条件：

粉末 Keep in dark place,Sealed in dry,Room Temperature

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 105 mg/mL(497.23 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 Dimethoxy-2-nitrobenzyl adenosine-3',5' cyclic monophosphate (DMNB) is a metabolically active, photolabile cyclic AMP analog that yields free cyclic AMP upon UV hydrolysis. The analog is useful in that it permits short term, transient elevations of intracellular cyclic AMP. Addition of DMNB (1-10 microM) to mouse mammary epithelial cells, followed by UV irradiation of cells, caused a significant increase in DNA synthesis over that observed with controls, UV irradiation alone or DMNB alone[2]. HA-DMNB (hyaluronan) is more resistant to hyaluronidase than HA, and HA-DMNB could release free carboxyl groups of HA upon ultraviolet to bond with H2O. More importantly, HA-DMNB was protective against UV light[3]. Moreover, a photocaged derivative (HP-DMNB) of HP (Heparin) was synthesized for light control of its anticoagulant activity. The synthesized HP-DMNB was characterized by NMR and FTIR analysis, which confirmed the successful modification of HP with the photocleavable 4,5-dimethoxyl-2-nitrobenzyl (DMNB) groups. After the modification, the molecular weight of HP-DMNB (DS = 0.34%) changed from 61 to 71 kDa. Anticoagulant activity analysis showed that HP-DMNB had a reduced anticoagulant capacity compared with commercial HP, while its anticoagulant activity will regain after releasing the free carboxyl groups of HP under UV light[4].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

4.74mL

0.95mL

0.47mL

23.68mL

4.74mL

2.37mL

47.36mL

9.47mL

4.74mL
参考文献

[2]Yuh IS, Sheffield LG. Photoreactive (caged) cyclic AMP analogs induce DNA synthesis in mammary epithelial cells. Cell Biol Int. 1995 Jul;19(7):603-8

[3]Sun F, Niu H, Wang D, Wu Y, Mu H, Ma L, Duan J. Novel moisture-preserving derivatives of hyaluronan resistant to hyaluronidase and protective to UV light. Carbohydr Polym. 2017 Feb 10;157:1198-1204

[4]Sun F, Liu Y, Wang D, Wang Z, Mu H, Wang F, Ding K, Duan J. A novel photocleavable heparin derivative with light controllable anticoagulant activity. Carbohydr Polym. 2018 Mar 15;184:191-198