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Glycyrrhizic acid

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Chemical Structure| 1405-86-3 同义名 : Glycyrrhizin;NSC 167409;Liquorice;Glizigen
CAS号 : 1405-86-3
货号 : A499217
分子式 : C42H62O16
纯度 : 80% HPLC
分子量 : 822.932
MDL号 : -
存储条件：

粉末 Inert atmosphere,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 105 mg/mL(127.59 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：

2% DMSO+30% PEG 300+2% Tween 80+water 10 mg/mL

生物活性
靶点

  • MAO

    MAO, IC50:0.16 μM
描述 Monoamine oxidases (MAO) are a group of enzymes that inactivate monoamine neurotransmitters and catalyze the oxidation of monoamines ingested in food. Glycyrrhizic acid (GA) is a MAO inhibitor with an IC50 value of 0.16 μM[3]. High mobility group box 1 (HMGB1) is a ubiquitous nuclear protein that can be released in response to necrotic or inflammatory signals. GA is also an inhibitor of HMGB1. In HBV transgenic mouse, GA administration (10 mg/mouse) ameliorated the severity of cytotoxic T lymphocytes‐induced liver disease[4]. GA also competitively inhibits the enzymatic activity of 11-hydroxysteroid dehydrogenase type 2 (11-HSD2). By treating rats with 60 and 120 mg/kg GA (twice a day) for 2 weeks, the level of NAD+-dependent 11-HSD2 activity in kidney was significantly decreased to 44% and 34%, respectively, as compared to control group. A significant reduction in renal 11-HSD2 protein and mRNA expressions was also observed in rats dosed with 120 mg/kg GA when compared to vehicle-treated group[5].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

1.22mL

0.24mL

0.12mL

6.08mL

1.22mL

0.61mL

12.15mL

2.43mL

1.22mL
参考文献

[1]Gong G, Xiang L, et al. Protective effect of glycyrrhizin, a direct HMGB1 inhibitor, on focal cerebral ischemia/reperfusion-induced inflammation, oxidative stress, and apoptosis in rats. PLoS One. 2014 Mar 4;9(3):e89450.

[2]Tanahashi T, Mune T, et al. Glycyrrhizic acid suppresses type 2 11 beta-hydroxysteroid dehydrogenase expression in vivo. J Steroid Biochem Mol Biol. 2002 Apr;80(4-5):441-7.

[3]Hatano T, Fukuda T, Miyase T, Noro T, Okuda T. Phenolic constituents of licorice. III. Structures of glicoricone and licofuranone, and inhibitory effects of licorice constituents on monoamine oxidase. Chem Pharm Bull (Tokyo). 1991 May;39(5):1238-43. doi: 10.1248/cpb.39.1238. PMID: 1913999.

[4]Sitia G, Iannacone M, Müller S, Bianchi ME, Guidotti LG. Treatment with HMGB1 inhibitors diminishes CTL-induced liver disease in HBV transgenic mice. J Leukoc Biol. 2007 Jan;81(1):100-7. doi: 10.1189/jlb.0306173. Epub 2006 Aug 25. PMID: 16935945.

[5]Tanahashi T, Mune T, Morita H, Tanahashi H, Isomura Y, Suwa T, Daido H, Gomez-Sanchez CE, Yasuda K. Glycyrrhizic acid suppresses type 2 11 beta-hydroxysteroid dehydrogenase expression in vivo. J Steroid Biochem Mol Biol. 2002 Apr;80(4-5):441-7. doi: 10.1016/s0960-0760(02)00033-x. PMID: 11983491.