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Basic Orange 14

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Chemical Structure| 10127-02-3 同义名 : Acridine Orange zinc chloride salt;Euchrysine 3RX
CAS号 : 10127-02-3
货号 : A479198
分子式 : C17H20Cl3N3Zn
纯度 : for electrophoresis
分子量 : 438.129
MDL号 : MFCD00081043
存储条件:

粉末 Sealed in dry,Room Temperature

液体 -20°C:3-6个月-80°C:12个月

溶解度 : -
动物实验配方:
生物活性
描述 Acridine Orange, zinc chloride salt, known as Euchrysine 3RX, is a nucleic acid-selective fluorescent dye that permeates cells. It emits orange fluorescence upon binding to ssDNA or RNA, and green fluorescence upon binding to dsDNA, with an excitation wavelength of 488 nm and emission wavelengths of green fluorescence at 530 nm and orange fluorescence at 640 nm[1][2][3].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.28mL

0.46mL

0.23mL

11.41mL

2.28mL

1.14mL

22.82mL

4.56mL

2.28mL

参考文献

[1]McMaster GK, et al. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarosegels by using glyoxal and acridine orange. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835-8.

[2]Traganos F, et al. Simultaneous staining of ribonucleic and deoxyribonucleic acids in unfixed cells using acridine orange in a flow cytofluorometric system. J Histochem Cytochem. 1977 Jan;25(1):46-56.

[3]Byvaltsev VA, et al. Acridine Orange: A Review of Novel Applications for Surgical Cancer Imaging and Therapy. Front Oncol. 2019 Sep 24;9:925.