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5-FAM SE

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Chemical Structure| 92557-80-7 同义名 : 5-Carboxyfluorescein N-Succinimidyl Ester;CFSE;5-FAM N-succinimidyl ester;5-Carboxyfluorescein-N-hydroxysuccinimide ester;5-Carboxyfluorescein NHS ester
CAS号 : 92557-80-7
货号 : A437441
分子式 : C25H15NO9
纯度 : 97%
分子量 : 473.388
MDL号 : MFCD00077322
存储条件：

粉末 Inert atmosphere,Store in freezer, under -20°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 25 mg/mL(52.81 mM)，配合低频超声，并水浴加热至45℃助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 5-FAM SE is a single isomer, serves as a fluorescent labeling agent for peptides, proteins, and nucleotides. This reagent reacts with amines to form stable amine conjugates, making it versatile for a variety of biochemical applications[1].[2].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.11mL

0.42mL

0.21mL

10.56mL

2.11mL

1.06mL

21.12mL

4.22mL

2.11mL
参考文献

[1]Sheng-da Qi, et al. Quantification of luminally released serotonin in rat proximal colon by capillary electrophoresis with laser-induced fluorescence detection. Anal Bioanal Chem. 2009 Apr;393(8):2059-66.

[2]Xiao-Ling Zhang, et al. Sensitive determination of pheomelanin after 5-carboxyfluorescein succinimidyl ester precapillary derivatization and micellar electrokinetic capillary chromatography with laser-induced fluorescence detection. J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Jan 1;861(1):136-9.