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Resveratrol

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Chemical Structure| 501-36-0 同义名 : trans-Resveratrol;SRT501;Vineatrol 20M.;Resvida;CA1201;RM1812;(E)-Resveratrol
CAS号 : 501-36-0
货号 : A419526
分子式 : C14H12O3
纯度 : 98%
分子量 : 228.24
MDL号 : MFCD00133799
存储条件：

粉末 Inert atmosphere,Store in freezer, under -20°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 105 mg/mL(460.04 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO

无水乙醇: 45 mg/mL(197.16 mM)，配合低频超声助溶，注意：无水乙醇开封后，易挥发，也会吸收空气中的水分，导致溶解能力下降，请避免使用开封较久的乙醇
动物实验配方：

5% DMSO+30% PEG 300+water 15 mg/mL

生物活性
靶点

  • SIRT1

  • JNK1

    JNK1, IC50:50 μM
描述 Resveratrol inhibits the activity of Cyclooxygenase, lipooxygenase, PKCs and p56, ERK1, JNK1, p38, IKK β, Src, STAT3, Ribonucleotide Reductase, DNA polymerases α and δ, PKD, PKC α, Quinone reductase 2, and Aromatase with IC50 of 0.035-60 μM. Resveratrol is also an activator of Adenylyl cyclase and AMPK with EC50 of 0.8 μM and 50 μM, respectively. The roles of Resveratrol as inhibitor or activator enable its effects on decreasing cell inflammatory associated behaviors, growth inhibition and induction of apoptosis in cancer cells, reversal of endothelin-1 stimulated cell responses, inhibition of phorbol ester-induced expression of COX-2, inhibition of DNA synthesis in cell, resistance to menadione-induced cell death, and improvement of cell mitochondrial function and glucose/lipid metabolism.
Resveratrol is also an activator of sirtuins. Resveratrol lowers the Michaelis constant of SIRT1 for both the acetylated substrate and NAD, and increases cell survival by stimulating SIRT1-dependent deacetylation of p53. In yeast, Resveratrol mimics calorie restriction by stimulating Sir2, increasing DNA stability and extending lifespan.
Resveratrol is effective at protecting isolated rat hearts against ischemia/reperfusion injury via its antioxidant activity, with improved recovery of developed pressure and aortic flow, reduction of malondialdehyde concentrations and reduction of infarct size.[1][2]
细胞研究
细胞系 浓度 检测类型 检测时间 活动说明 数据源
HEK293 cells Function assay 48 h Inhibition of TNF-alpha-induced NF-kappaB activity expressed in HEK293 cells after 48 hrs by luciferase reporter gene assay, IC50=0.173 μM 20527891
HEK293T cells Function assay Inhibition of TNF-alpha-induced NF-kappaB activation in HEK293T cells, IC50=20 μM 17125270
human 293 cells Function assay 24 h Inhibition of TNFalpha induced NF-kappaB activation in human 293 cells after 24 hrs by luciferase reporter gene assay, IC50=16.1 μM 18487053
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

4.38mL

0.88mL

0.44mL

21.91mL

4.38mL

2.19mL

43.81mL

8.76mL

4.38mL
参考文献

[1]Pirola L, Frojdo S. Resveratrol: one molecule, many targets. IUBMB Life. 2008 May;60(5):323-32.

[2]Howitz KT, Bitterman KJ, et al. Small molecule activators of sirtuins extend Saccharomyces cerevisiae lifespan. Nature. 2003 Sep 11;425(6954):191-6.

[3]Zunino SJ, Storms DH, et al. Resveratrol given intraperitoneally does not inhibit the growth of high-risk t(4;11) acute lymphoblastic leukemia cells in a NOD/SCID mouse model. Int J Oncol. 2012;40(4):1277-84.

[4]Thirunavukkarasu M, Penumathsa SV, et al. Resveratrol alleviates cardiac dysfunction in streptozotocin-induced diabetes: Role of nitric oxide, thioredoxin, and heme oxygenase. Free Radic Biol Med. 2007;43(5):720-9.

[5]Harper CE, Cook LM, et al. Genistein and resveratrol, alone and in combination, suppress prostate cancer in SV-40 tag rats. Prostate. 2009;69(15):1668-82.

[6]Singh M, Parent S, et al. Resveratrol modulates the expression of PTGS2 and cellular proliferation in the normal rat endometrium in an AKT-dependent manner. Biol Reprod. 2011;84(5):1045-52.