Epothilone A

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Chemical Structure| 152044-53-6 同义名 : 西洛他唑A ;Epo A
CAS号 : 152044-53-6
货号 : A408460
分子式 : C26H39NO6S
纯度 : 98%
分子量 : 493.656
MDL号 : MFCD26960884
存储条件：

Pure form Keep in dark place,Sealed in dry,Store in freezer, under -20°C

In solvent -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 120 mg/mL(243.08 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 Epothilone A competitively inhibits the binding of [3H] paclitaxel to tubulin polymers. The apparent Ki value for Epothilone A is 1.4 μM according to Hanes analysis and 0.6 μM according to Dixon analysis[1]. Epothilone A demonstrates high cytotoxicity (IC50=0.05 μM) in vitro against the human T-24 bladder carcinoma cell line. In competition assays, the binding affinity of Epothilone A to tubulin is comparable to that of paclitaxel. The IC50 values for displacing 100 nM of (3H) paclitaxel from the tubulin binding site are 3.6 μM for paclitaxel, 2.3 μM for Epothilone A, and 3.3 μM for patupilone[2].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.03mL

0.41mL

0.20mL

10.13mL

2.03mL

1.01mL

20.26mL

4.05mL

2.03mL
参考文献

[1]Kowalski RJ, et al. Activities of the microtubule-stabilizing agents epothilones A and B with purified tubulin and in cells resistant to paclitaxel (Taxol(R)). J Biol Chem. 1997 Jan 24;272(4):2534-41.

[2]Cheng KL, et al. Novel microtubule-targeting agents - the epothilones. Biologics. 2008 Dec;2(4):789-811.