产品说明书
DIM-C-pPhOH
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同义名 : | - |
| CAS号 : | 151358-47-3 | |
| 货号 : | A386994 | |
| 分子式 : | C23H18N2O | |
| 纯度 : | 98%+ | |
| 分子量 : | 338.402 | |
| MDL号 : | MFCD03723253 | |
| 存储条件： |
粉末 Keep in dark place,Sealed in dry,Room Temperature 液体 -20°C:3-6个月-80°C:12个月 |
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| 溶解度 : |
DMSO: 120 mg/mL(354.61 mM)，配合低频超声，并水浴加热至45℃助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO |
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| 动物实验配方： |
| 生物活性 | |||
|---|---|---|---|
| 描述 | The orphan nuclear receptor NR4A1 (nuclear receptor 4A1) exhibits pro-oncogenic activity in cancer cell lines. NR4A1 activates mTOR signaling, regulates genes such as thioredoxin domain containing 5 and isocitrate dehydrogenase 1 that maintain low oxidative stress, and coactivates specificity protein 1 (Sp1)-regulated pro-survival and growth promoting genes. DIM-C-pPhOH binds NR4A1 and act as antagonists. DIM-C-pPhOH (20 μM; 18 hr) inhibited growth and induced apoptosis in ACHN and 786-O cells, and the functional and genomic effects of it were comparable to those observed after NR4A1 knockdown. Moreover, treatment of these cells with 0–20 μM of the NR4A1 antagonist DIM-C-pPhOH also significantly decreased cell proliferation. IC50 values for DIM-C-pPhOH were 13.6 μM and 13.0 μM in ACHN cells and 786-O cells, respectively. Moreover, treatment of athymic nude mice bearing ACHN cells as xenografts with DIM-C-pPhOH (30 mg/kg/d) for 50 days also resulted in a significant inhibition of tumor growth and complemented results of the in vitro studies. In addition, researchers also observed decreased expression of survivin, bcl-2, EGFR and induced PARP cleavage in tumor lysates from nude mice bearing ACHN cells as a xenograft and treated with DIM-C-pPhOH (30 mg/kg/d)[1]. | ||
| 实验方案 | |||
|---|---|---|---|
| 1mg | 5mg | 10mg | |
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1 mM 5 mM 10 mM |
2.96mL 0.59mL 0.30mL |
14.78mL 2.96mL 1.48mL |
29.55mL 5.91mL 2.96mL |
| 参考文献 |
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[1]Nuclear Receptor 4A1 (NR4A1) as a Drug Target for Renal Cell Adenocarcinoma |