Cucurbitacin B

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Chemical Structure| 6199-67-3 同义名 : Cuc B;NSC 144154;NSC 49451
CAS号 : 6199-67-3
货号 : A380132
分子式 : C32H46O8
纯度 : 99%
分子量 : 558.703
MDL号 : MFCD07778083
存储条件：

Pure form Keep in dark place,Sealed in dry,2-8°C

In solvent -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 105 mg/mL(187.94 mM)，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 Microtubule, a component of the cytoskeleton, plays crucial roles in various fundamental cell functions, including mitotic regulation, maintenance of cell morphology, intracellular motility and macromolecules and organelles trafficking. Cucurbitacin B, an oxygenated tetracyclic triterpenoid compound extracted from the Thai medicinal plant Trichosanthes cucumerina L., has several biological activities including anti-inflammatory, antimicrobial and anticancer through disruption of the microtubule network. It exhibited a dose-dependent inhibitory effect with the IC50 of 4.12 μM and 3.68 μM for MCF-7 and MDA-MB-231 cells, respectively. In the same cell lines treated with 2.5 μM and 5 μM cucurbitacin B for 24 hr, blockage at G2/M and apoptotic induction were observed in cucurbitacin B-treated cells. Further, cucurbitacin B treatment for 24 hr significantly induced apoptosis approximately 30% to 40% of breast cancer cells population. Compared with the control cells, cucurbitacin B treated cells exhibited obvious aggregation of α-tubulin. The changes were observed within 15 min during incubation with cucurbitacin B[3]. In the nude mice bearing human MDA-MB-231 breast cancer cells, tumor volume was reduced by 55% in the group that received Cucurbitacin B (1.0 mg/kg) for 6 weeks compared with vehicle controls[4].
临床研究
NCT号 适应症或疾病 临床期 招募状态 预计完成时间 地点
NCT02858388 Otitis Media With Effusion Phase 3 Completed - Italy ... 展开 >> San Gennaro Hospital Naples, Italy, 80136 收起 <<
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

1.79mL

0.36mL

0.18mL

8.95mL

1.79mL

0.89mL

17.90mL

3.58mL

1.79mL
参考文献

[1]Gupta P, Srivastava SK. Inhibition of Integrin-HER2 signaling by Cucurbitacin B leads to in vitro and in vivo breast tumor growth suppression. Oncotarget. 2014 Apr 15;5(7):1812-28.

[2]Shang Y, Guo XX, et al. Cucurbitacin-B inhibits neuroblastoma cell proliferation through up-regulation of PTEN. Eur Rev Med Pharmacol Sci. 2014;18(21):3297-303.

[3]Duangmano S, Sae-Lim P, Suksamrarn A, Domann FE, Patmasiriwat P. Cucurbitacin B inhibits human breast cancer cell proliferation through disruption of microtubule polymerization and nucleophosmin/B23 translocation. BMC Complement Altern Med. 2012;12:185

[4]Wakimoto N, Yin D, O'Kelly J, et al. Cucurbitacin B has a potent antiproliferative effect on breast cancer cells in vitro and in vivo. Cancer Sci. 2008;99(9):1793-1797