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KY02111

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Chemical Structure| 1118807-13-8 同义名 : -
CAS号 : 1118807-13-8
货号 : A360800
分子式 : C18H17ClN2O3S
纯度 : 99%+
分子量 : 376.857
MDL号 : MFCD08483062
存储条件：

粉末 Sealed in dry,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 35 mg/mL(92.87 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 Wnt signaling is one of the key cascades regulating development and stemness, and has also been tightly associated with cancer[6]. IWP-4 is a small molecule Wnt inhibitor with an IC50 of 25 nM[7]. IWP-4 treatment resulted in a large percentage of cells expressing low amounts of cardiac myosin heavy chain and expression of early cardiac progenitor markers ISL1 and NKX2-5, thus indicating the production of large numbers of immature cardiomyocytes (~65,000/cm2 or ~1.5 per input human embryonic stem cell (hESC)). In addition, IWP-4 induced expression of both atrial (MLC2a) and ventricular (MLC2v) forms[7]. Mesenchymal precursor cells (MPCs) treated with IWP-4 express elevates levels of DKK1 (Dickkopf homolog 1) and GSK3β (glycogen synthase kinase 3 β) on day 21. IWP-4 also causes a significant down regulation of SPARC (secreted protein acidic and rich incysteine) and COL1A1 (Collagen 1A1)[8].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.65mL

0.53mL

0.27mL

13.27mL

2.65mL

1.33mL

26.54mL

5.31mL

2.65mL
参考文献

[1]Minami I, Yamada K, et al. A small molecule that promotes cardiac differentiation of human pluripotent stem cells under defined, cytokine- and xeno-free conditions. Cell Rep. 2012 Nov 29;2(5):1448-60.

[2]Zhan T, Rindtorff N, Boutros M. Wnt signaling in cancer. Oncogene. 2017 Mar;36(11):1461-1473.

[3]Minami I, Yamada K, Otsuji TG, Yamamoto T, Shen Y, Otsuka S, Kadota S, Morone N, Barve M, Asai Y, Tenkova-Heuser T, Heuser JE, Uesugi M, Aiba K, Nakatsuji N. A small molecule that promotes cardiac differentiation of human pluripotent stem cells under defined, cytokine- and xeno-free conditions. Cell Rep. 2012 Nov 29;2(5):1448-60.

[4]Morgan JT, Raghunathan VK, Chang YR, Murphy CJ, Russell P. Wnt inhibition induces persistent increases in intrinsic stiffness of human trabecular meshwork cells. Exp Eye Res. 2015 Mar;132:174-8.

[5]Surface LE, Fields PA, Subramanian V, Behmer R, Udeshi N, Peach SE, Carr SA, Jaffe JD, Boyer LA. H2A.Z.1 Monoubiquitylation Antagonizes BRD2 to Maintain Poised Chromatin in ESCs. Cell Rep. 2016 Feb 9;14(5):1142-1155.

[6]Zhan T, Rindtorff N, Boutros M. Wnt signaling in cancer. Oncogene. 2017 Mar;36(11):1461-1473. doi: 10.1038/onc.2016.304. Epub 2016 Sep 12. PMID: 27617575; PMCID: PMC5357762.

[7]Hudson J, Titmarsh D, Hidalgo A, Wolvetang E, Cooper-White J. Primitive cardiac cells from human embryonic stem cells. Stem Cells Dev. 2012 Jun 10;21(9):1513-23. doi: 10.1089/scd.2011.0254. Epub 2011 Nov 9. PMID: 21933026.

[8]Frith JE, Titmarsh DM, Padmanabhan H, Cooper-White JJ. Microbioreactor array screening of Wnt modulators and microenvironmental factors in osteogenic differentiation of mesenchymal progenitor cells. PLoS One. 2013 Dec 23;8(12):e82931. doi: 10.1371/journal.pone.0082931. PMID: 24376608; PMCID: PMC3871528.