产品说明书

Kaempferol-3-O-rutinoside

Print
Chemical Structure| 17650-84-9 同义名 : 山奈酚-3-O-芸香糖苷 ;Nicotiflorin;3,4′,5,7-Tetrahydroxyflavone 3-rhamnoglucoside;Kaempferol 3-O-β-rutinoside;Nikotoflorin
CAS号 : 17650-84-9
货号 : A347566
分子式 : C27H30O15
纯度 : 99%+
分子量 : 594.518
MDL号 : MFCD03427292
存储条件：

粉末 Inert atmosphere,Store in freezer, under -20°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 250 mg/mL(420.51 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 Nicotiflorin is a single component extracted from traditional Chinese medicine Flos Carthami. At doses of 2.5, 5 and 10 mg/kg, nicotiflorin administered immediately after the onset of ischemia markedly reduced brain infarct volume and neurological deficits. For primarily cultured neurons suffered 2 h hypoxia followed by 24 h reoxygenation, nicotiflorin significantly attenuated cell death and reduced LDH release[3]. In vivo, nicotiflorin treatment alleviated brain damage and neurological deficit while it dramatically increased 72 h survival rate in rats. In vitro, nicotiflorin treatment also ameliorated the severity of OGD/R(oxygen-glucose deprivation and reintroduction). In vitro, nicotiflorin likewise enhanced autophagy and promoted autophagy flux[4]. Nicotiflorin (10 mg/kg) protects against cerebral ischemia/reperfusion injury-induced apoptosis via the JAK2/STAT3 pathway[5]. Nicotiflorin and rutin showed neuroprotective effects on hypoxia-, glutamate- or oxidative stress-induced RGC (retinal ganglion cell) death at concentrations of 1 nM or higher[6].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

1.68mL

0.34mL

0.17mL

8.41mL

1.68mL

0.84mL

16.82mL

3.36mL

1.68mL
参考文献

[1]Kroner A, Marnet N, et al. Nicotiflorin, rutin and chlorogenic acid: phenylpropanoids involved differently in quantitative resistance of potato tubers to biotrophic and necrotrophic pathogens. Plant Physiol Biochem. 2012 Aug;57:23-31.

[2]Lee HB, Kim EK, et al. Isolation and characterization of nicotiflorin obtained by enzymatic hydrolysis of two precursors in tea seed extract. J Agric Food Chem. 2010 Apr 28;58(8):4808-13.

[3]Li R, Guo M, Zhang G, Xu X, Li Q. Neuroprotection of nicotiflorin in permanent focal cerebral ischemia and in neuronal cultures. Biol Pharm Bull. 2006 Sep;29(9):1868-72

[4]Wang Y, Zhang S, Ni H, Zhang Y, Yan X, Gao Y, He B, Wang W, Liu C, Guo M. Autophagy is involved in the neuroprotective effect of nicotiflorin. J Ethnopharmacol. 2021 Oct 5;278:114279

[5]Hu GQ, Du X, Li YJ, Gao XQ, Chen BQ, Yu L. Inhibition of cerebral ischemia/reperfusion injury-induced apoptosis: nicotiflorin and JAK2/STAT3 pathway. Neural Regen Res. 2017 Jan;12(1):96-102

[6]Nakayama M, Aihara M, Chen YN, Araie M, Tomita-Yokotani K, Iwashina T. Neuroprotective effects of flavonoids on hypoxia-, glutamate-, and oxidative stress-induced retinal ganglion cell death. Mol Vis. 2011;17:1784-93. Epub 2011 Jul 2