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WAY 316606

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Chemical Structure| 915759-45-4 同义名 : -
CAS号 : 915759-45-4
货号 : A333983
分子式 : C18H19F3N2O4S2
纯度 : 98+%
分子量 : 448.48
MDL号 : MFCD18206894
存储条件：

粉末 Keep in dark place,Inert atmosphere,Room temperature

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 105 mg/mL(234.12 mM)，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 The Wnt signaling pathway is activated by secreted Wnt glycoproteins and regulates various biological processes, including skeletal development. Secreted frizzled-related protein-1 (sFRP-1) is a negative regulator or an antagonist of the Wnt signaling pathway. WAY-316606 is identified as an inhibitor of sFRP-1 with IC50 value of 0.5 μM[3]. WAY-316606 enhanced total bone area up to 60% in a dose-dependent manner when tested in a neonatal murine ex vivo calvarial assay[3]. WAY-316606 treated rat mandibles (1 mM, 10 uL, on alternate days for 7 days) showed a substantial gain of interradicular bone mass near the inhibitor application site compared to rat mandibles treated with a vehicle control, in MicroCT analysis[4]. Differentiating periodontal ligament cells cultured with 1 and 2 uM of WAY-316606 showed increased alkaline phosphatase activity and enhanced mineral deposits in a dose dependent manner compared to cells cultured with induction media alone[4].
作用机制 WAY-316606 inhibits sFRP-1 perhaps via the netrin domain.
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.23mL

0.45mL

0.22mL

11.15mL

2.23mL

1.11mL

22.30mL

4.46mL

2.23mL
参考文献

[1]Moore WJ, Kern JC, et al. Modulation of Wnt signaling through inhibition of secreted frizzled-related protein I (sFRP-1) with N-substituted piperidinyl diphenylsulfonyl sulfonamides. J Med Chem. 2009 Jan 8;52(1):105-16.

[2]Bodine PV, Stauffer B, et al. A small molecule inhibitor of the Wnt antagonist secreted frizzled-related protein-1 stimulates bone formation. Bone. 2009 Jun;44(6):1063-8.

[3]Moore WJ, Kern JC, Bhat R, Commons TJ, Fukayama S, Goljer I, Krishnamurthy G, Magolda RL, Nogle L, Pitts K, Stauffer B, Trybulski EJ, Welmaker GS, Wilson M, Bodine PV. Modulation of Wnt signaling through inhibition of secreted frizzled-related protein I (sFRP-1) with N-substituted piperidinyl diphenylsulfonyl sulfonamides. J Med Chem. 2009 Jan 8;52(1):105-16. doi: 10.1021/jm801144h. PMID: 19072540.

[4]Gopinathan G, Foyle D, Luan X, Diekwisch TGH. The Wnt Antagonist SFRP1: A Key Regulator of Periodontal Mineral Homeostasis. Stem Cells Dev. 2019 Aug 1;28(15):1004-1014. doi: 10.1089/scd.2019.0124. Epub 2019 Jul 22. PMID: 31215318; PMCID: PMC6661921.