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IWP-4

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Chemical Structure| 686772-17-8 同义名 : Inhibitor of Wnt Production-4
CAS号 : 686772-17-8
货号 : A313997
分子式 : C23H20N4O3S3
纯度 : 99%+
分子量 : 496.625
MDL号 : MFCD04457754
存储条件：

粉末 Inert atmosphere,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 1 mg/mL(2.01 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 IWP-4 is a potent Wnt pathway inhibitor with an IC50 of 25 nM, promoting the expression of cardiac indicators such as cardiac troponin I (CTNI) and bright cells expressing cardiac myosin heavy chain (MYHhi+). It leads to the emergence of beating foci (0.44±0.10 SEM beats per second), not observed in cultures without IWP-4 treatment. Flow cytometric analysis reveals a significant increase in MYHlo+ cells in IWP-4 treated cultures compared to untreated ones at day 16, with percentages of 17.0±1.3 SD% and 5.4±1.4 SD%, respectively. Additionally, 63% (481/817) of cells treated with IWP-4 show nuclear NKX2-5 protein expression[1]. Mesenchymal precursor cells (MPCs) exposed to IWP-4 do not exhibit significant differences in AXIN2, CTNNB1, and GSK3B expression levels compared to those treated with osteogenic medium alone on day 7. However, by day 21, MPCs treated with IWP-4 show increased levels of DKK1 and GSK3β. IWP-4 also significantly reduces the expression of SPARC and COL1A1[2].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.01mL

0.40mL

0.20mL

10.07mL

2.01mL

1.01mL

20.14mL

4.03mL

2.01mL
参考文献

[1]Hudson J, et al. Primitive cardiac cells from human embryonic stem cells. Stem Cells Dev. 2012 Jun 10;21(9):1513-23.

[2]Frith JE, et al. Microbioreactor array screening of Wnt modulators and microenvironmental factors in osteogenic differentiation of mesenchymal progenitor cells. PLoS One. 2013 Dec 23;8(12):e82931.