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Kaurenoic acid

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Chemical Structure| 6730-83-2 同义名 : 贝壳杉烯酸 ;kaurenoate;kaur-16-en-18-oic acid;Kauren-19-oic acid;CCRIS 1514
CAS号 : 6730-83-2
货号 : A303353
分子式 : C20H30O2
纯度 : 99%+
分子量 : 302.451
MDL号 : MFCD03700717
存储条件：

粉末 Sealed in dry,Store in freezer, under -20°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 105 mg/mL(347.16 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 Kaurenoic acid (KA) is a diterpene from Sphagneticola trilobata, inhibits Inflammatory Pain by the inhibition of cytokine production and activation of the NO–cyclic GMP–PKG–ATP-sensitive potassium channel signaling pathway[3]. Kaurenoic acid dose-dependently inhibited NO production, PGE2 release, COX-2 and iNOS expression at micromolar concentrations in LPS-induced RAW264.7 macrophages with IC50 values of 51.73 (±2.42) μM and 106.09 (±0.27) μM in NO production and PGE(2) release, respectively[4]. KA induced the phosphorylation of Smad2 and Smad3, key activator molecules in TGF-β signaling. Similarly, i.t. KA induced the phosphorylation of Smad2 and increased the expression of α-SMA in mouse lungs[5]. KA increased, in a dose-dependent manner, the survival rate after acetaminophen overdose. KA reduced acetaminophen-induced hepatic necrosis and ALT and AST levels. KA decreased acetaminophen-induced neutrophil and macrophage recruitment, oxidative stress and the production of IL-33, TNF-α and IL-1β, alongside with normalisation of IL-10 levels in the liver[6].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

3.31mL

0.66mL

0.33mL

16.53mL

3.31mL

1.65mL

33.06mL

6.61mL

3.31mL
参考文献

[1]Fernandes VC, Pereira SI, et al. The epimer of kaurenoic acid from Croton antisyphiliticus is cytotoxic toward B-16 and HeLa tumor cells through apoptosis induction. Genet Mol Res. 2013 Apr 2;12(2):1005-11.

[2]Choi RJ, Shin EM, et al. Inhibitory effects of kaurenoic acid from Aralia continentalis on LPS-induced inflammatory response in RAW264.7 macrophages. Phytomedicine. 2011 Jun 15;18(8-9):677-82.

[3]Mizokami SS, Arakawa NS, Ambrosio SR, Zarpelon AC, Casagrande R, Cunha TM, Ferreira SH, Cunha FQ, Verri WA Jr. Kaurenoic acid from Sphagneticola trilobata Inhibits Inflammatory Pain: effect on cytokine production and activation of the NO-cyclic GMP-protein kinase G-ATP-sensitive potassium channel signaling pathway. J Nat Prod. 2012 May 25;75(5):896-904

[4]Choi RJ, Shin EM, Jung HA, Choi JS, Kim YS. Inhibitory effects of kaurenoic acid from Aralia continentalis on LPS-induced inflammatory response in RAW264.7 macrophages. Phytomedicine. 2011 Jun 15;18(8-9):677-82

[5]Kim KH, Han JW, Jung SK, Park BJ, Han CW, Joo M. Kaurenoic acid activates TGF-β signaling. Phytomedicine. 2017 Aug 15;32:8-14

[6]Marcondes-Alves L, Fattori V, Borghi SM, Lourenco-Gonzalez Y, Bussmann AJC, Hirooka EY, Casagrande R, Verri WA Jr, Arakawa NS. Kaurenoic acid extracted from Sphagneticola trilobata reduces acetaminophen-induced hepatotoxicity through inhibition of oxidative stress and pro-inflammatory cytokine production in mice. Nat Prod Res. 2019 Mar;33(6):921-924