产品说明书
ESI-05
 |
同义名 : | NSC 116966 |
| CAS号 : | 5184-64-5 | |
| 货号 : | A257124 | |
| 分子式 : | C16H18O2S | |
| 纯度 : | 98% | |
| 分子量 : | 274.378 | |
| MDL号 : | MFCD00091573 | |
| 存储条件： |
粉末 Sealed in dry,Room Temperature 液体 -20°C:3-6个月-80°C:12个月 |
|
| 溶解度 : |
DMSO: 50 mg/mL(182.23 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO |
|
| 动物实验配方： |
| 生物活性 | |||
|---|---|---|---|
| 描述 | ESI 05 is a selective EPAC2 inhibitor. It inhibits cAMP binding to EPAC2 and cAMP mediated EPAC2 guanine nucleotide exchange factor (GEF) activity (IC50=0.4μM)[1]. It has been tested the ability of ESI 05 to compete with the binding of 8-NBD-cAMP to EPAC2. ESI-05 showed an increased potency with apparent IC50 of 0.4 ± 0.1μM, representing a 100- or 67-fold increase in affinity over cAMP[2]. ESI-05 (1, 5, 10, and 25 µM) reduces activation of the GTPase RAP1 induced by the Epac-selective cAMP analog 007-AM in HEK293 cells expressing Epac2 but has no effect in HEK293 cells expressing Epac1. | ||
| 实验方案 | |||
|---|---|---|---|
| 1mg | 5mg | 10mg | |
|
1 mM 5 mM 10 mM |
3.64mL 0.73mL 0.36mL |
18.22mL 3.64mL 1.82mL |
36.45mL 7.29mL 3.64mL |
| 参考文献 |
|---|
|
[1]Rehmann, H. Epac-inhibitors: Facts and Artefacts. Sci Rep. 2013. 3032(3). |