产品说明书

Catharanthine

Print
Chemical Structure| 2468-21-5 同义名 : (+)-3,4-Didehydrocoronaridine
CAS号 : 2468-21-5
货号 : A247451
分子式 : C21H24N2O2
纯度 : 98%
分子量 : 336.428
MDL号 : MFCD01753356
存储条件：

粉末 Sealed in dry,Store in freezer, under -20°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 105 mg/mL(312.1 mM)，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
靶点

  • nAChR
描述 Catharanthine is a constituent of anticancer vinca alkaloids. Catharanthine evoked concentration-dependent decreases (I(max) >98%) in endothelium-independent tonic responses of aortic rings to phenylephrine (PE) and KCl (IC50 = 28 µM for PE and IC50 = 34 µM for KCl) and of third-order branches of the small mesenteric artery (MA) (IC50 = 3 µM for PE and IC50 = 6 µM for KCl). Catharanthine also increased the inner vessel wall diameter (IC50 = 10 µM) and reduced intracellular free Ca2+ levels (IC50 = 16 µM) in PE-constricted MAs. Patch-clamp studies demonstrated that catharanthine inhibited voltage-operated L-type Ca2+ channel (VOCC) currents in cardiomyocytes and VSMCs (IC50 = 220 µM and IC50 = 8 µM, respectively) of MA[3].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.97mL

0.59mL

0.30mL

14.86mL

2.97mL

1.49mL

29.72mL

5.94mL

2.97mL
参考文献

[1]Pereira DM, Ferreres F, et al. Pharmacological effects of Catharanthus roseus root alkaloids in acetylcholinesterase inhibition and cholinergic neurotransmission. Phytomedicine. 2010 Jul;17(8-9):646-52.

[2]Prakash V, Timasheff SN. Mechanism of interaction of vinca alkaloids with tubulin: catharanthine and vindoline. Biochemistry. 1991 Jan 22;30(3):873-80.

[3]Jadhav A, Liang W, Papageorgiou PC, Shoker A, Kanthan SC, Balsevich J, Levy AS, Heximer S, Backx PH, Gopalakrishnan V. Catharanthine dilates small mesenteric arteries and decreases heart rate and cardiac contractility by inhibition of voltage-operated calcium channels on vascular smooth muscle cells and cardiomyocytes. J Pharmacol Exp Ther. 2013 Jun;345(3):383-92.