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Diphenyleneiodonium chloride

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Chemical Structure| 4673-26-1 同义名 : 二苯基氯化碘盐 ;DPI;Diphenyleneiodonium (chloride)
CAS号 : 4673-26-1
货号 : A246246
分子式 : C12H8ClI
纯度 : 95%
分子量 : 314.549
MDL号 : MFCD00214165
存储条件：

粉末 Keep in dark place,Inert atmosphere,Store in freezer, under -20°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 5 mg/mL(15.9 mM)，配合低频超声，并水浴加热至45℃助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：

3% DMSO+40% PEG 300+2% Tween 80+water 0.5 mg/mL

生物活性
描述 Transient receptor potential ankyrin 1 (TRPA1) is a Ca2+-permeable nonselective cation channel expressed in neuronal and nonneuronal cells and plays an important role in acute and inflammatory pain. Diphenyleneiodonium chloride (DPI), a NADPH oxidase (NOX) inhibitor, also functions as a TRPA1 activator with an EC50 of 1 to 3 μM. Application of DPI at 0.03-10 μM induced a Ca2+ response in HEK-TRPA1 cells in a concentration-dependent manner. In inflammatory human synoviocytes where TRPA1 is functionally expressed, 3 μM DPI evoked a Ca2+ response when cells were treated with IL1α suggesting that DPI activates endogenous TRPA1 expressed in human synoviocytes[3]. In human RPE cell line ARPE-19 cells, DPI (0.1, 1, and 10 μM) resulted in a significant decrease of cell proliferation, without vacuolation and detachment of the cells from the dish. Significantly increased level of p53 was detected at 4 h after treatment of DPI[4]. In mice, intraplantar injection of 2 mM DPI caused a pain-related response which was inhibited by preadministration with HC (a selective TRPA1 antagonist, HC-030031)[3].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

3.18mL

0.64mL

0.32mL

15.90mL

3.18mL

1.59mL

31.79mL

6.36mL

3.18mL
参考文献

[1]Pandey M, Singh AK, et al. Diphenyleneiodonium chloride (DPIC) displays broad-spectrum bactericidal activity. Sci Rep. 2017 Sep 14;7(1):11521.

[2]Osaki T, Uchida Y, et al. Diphenyleneiodonium chloride, an inhibitor of reduced nicotinamide adenine dinucleotide phosphate oxidase, suppresses light-dependent induction of clock and DNA repair genes in zebrafish. Biol Pharm Bull. 2011;34(8):1343-7.

[3]Suzuki H, Hatano N, Muraki Y, Itoh Y, Kimura S, Hayashi H, Onozaki K, Ohi Y, Haji A, Muraki K. The NADPH oxidase inhibitor diphenyleneiodonium activates the human TRPA1 nociceptor. Am J Physiol Cell Physiol. 2014 Aug 15;307(4):C384-94.

[4]Park SE, Song JD, Kim KM, Park YM, Kim ND, Yoo YH, Park YC. Diphenyleneiodonium induces ROS-independent p53 expression and apoptosis in human RPE cells. FEBS Lett. 2007 Jan 23;581(2):180-6.