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Chemical Structure| 152918-18-8 同义名 : IB-MECA;CF-101;ALB-7208
CAS号 : 152918-18-8
货号 : A238381
分子式 : C18H19IN6O4
纯度 : 99%+
分子量 : 510.286
MDL号 : MFCD00671785
存储条件：

粉末 Keep in dark place,Sealed in dry,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 45 mg/mL(88.19 mM)，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 Adenosine receptors play an important role in cell growth and proliferation in different cell lines. The A3 adenosine receptor (A3AR) expression level in normal cells is low, while it is more highly expressed in various tumor cells. IB-MECA is a reversable A3AR agonist, inhibiting the growth of different cancer cell types like melanoma, colon, breast, leukemia, and prostate. IB-MECA was able to inhibit forskolin-stimulated cyclic adenosine monophosphate (cAMP) levels with EC50s of 0.82 μM and 1.2 μM in OVCAR-3 and Caov-4 ovarian cancer cells. Further, IB-MECA exhibited antiproliferative effect with IC50s of 32.14 μM and 45.37 μM for OVCAR-3 and Caov-4 ovarian cancer cells, respectively. 48 h treatment with IB-MECA induced apoptosis in those cell lines with dosage range 0.1-100 μM and significantly reduced cell viability in a dose-dependent manner with dosage range 0.0001-100 μM[3]. In B10CBAF1 male mice aged 3 months, IB-MECA (105 μg/kg) resulted in statistically significant increases of the mean survival time in comparison with the control irradiated mice[4].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

1.96mL

0.39mL

0.20mL

9.80mL

1.96mL

0.98mL

19.60mL

3.92mL

1.96mL
参考文献

[1]Abedi H, Aghaei M, et al. Mitochondrial and caspase pathways are involved in the induction of apoptosis by IB-MECA in ovarian cancer cell lines. Tumour Biol. 2014 Nov;35(11):11027-39.

[2]Hofer M, Pospisil M, et al. Agonist of the adenosine A3 receptor, IB-MECA, and inhibitor of cyclooxygenase-2, meloxicam, given alone or in a combination early after total body irradiation enhance survival of γ-irradiated mice. Radiat Environ Biophys. 2014 Mar;53(1):211-215.

[3]Abedi H, et al. Mitochondrial and caspase pathways are involved in the induction of apoptosis by IB-MECA in ovarian cancer cell lines. Tumour Biol. 2014 Nov;35(11):11027-39

[4]Shin Y, et al. Activation of Phosphoinositide Breakdown and Elevation of Intracellular Calcium in a Rat RBL-2H3 Mast Cell Line by Adenosine Analogs: Involvement of A(3)-Adenosine Receptors? Drug Dev Res. 1996 Sep 1;39(1):36-46