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Glycitein

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Chemical Structure| 40957-83-3 同义名 : Glycetein
CAS号 : 40957-83-3
货号 : A231798
分子式 : C16H12O5
纯度 : 99%+
分子量 : 284.264
MDL号 : MFCD00016679
存储条件：

粉末 Sealed in dry,Store in freezer, under -20°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 25 mg/mL(87.95 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 Glycitein, as one of the three major isoflavones in soybeans, directly but significantly (about 5%) suppressed the proliferation of MC3T3-E1 and stimulated bone-related protein (alkaline phosphatase (ALP) and osteocalcin (OC)) expression[3]. Glycitein has weak estrogenic activity, comparable to that of the other soy isoflavones but much lower than that of DES and 17beta-estradiol[4]. Glycitein exhibits biphasic regulation on SKBR-3 cells. At concentrations of less than 10 mg/mL, cells respond to glycitein by increasing cell growth and de novo DNA synthesis whereas the addition of glycitein at concentrations greater than 30 mg/mL significantly inhibited cell growth and DNA synthesis in a dose-dependent manner. Glycitein was found to be cytostatic at low concentrations and cytotoxic at higher concentrations. Treatment with 100 mg/mL of glycitein severely altered the cell morphology[5]. Osteoclast generation was inhibited by glycitein in a biphasic-dose-dependent manner and showed the greatest inhibitory effects at 10 nM. Glycitein increased caspase 3/7 activity by 15% at a concentration of 10 nM. Further, 10 nM glycitein significantly decreased the expression of IL-6 and RANKL in osteoblasts but did not change mRNA levels of OPG(osteoprotegerin) [6].
临床研究
NCT号 适应症或疾病 临床期 招募状态 预计完成时间 地点
NCT01232751 Postmenopause Not Applicable Completed - Netherlands ... 展开 >> Wageningen University Wageningen, Gelderland, Netherlands, 6703 HD 收起 <<
NCT03008785 Bloodpressure Not Applicable Completed - Brazil ... 展开 >> Guilherme Morais Puga Uberlandia, Minas Gerais, Brazil, 38400-678 收起 <<
NCT01048606 Overweight Phase 4 Completed - Canada, Quebec ... 展开 >> Centre de recherche sur le vieillissement du CSSS-IUGS Sherbrooke, Quebec, Canada, J1H 4C4 收起 <<
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

3.52mL

0.70mL

0.35mL

17.59mL

3.52mL

1.76mL

35.18mL

7.04mL

3.52mL
参考文献

[1]Pan W, Ikeda K, et al. Genistein, daidzein and glycitein inhibit growth and DNA synthesis of aortic smooth muscle cells from stroke-prone spontaneously hypertensive rats. J Nutr. 2001 Apr;131(4):1154-8.

[2]Song TT, Hendrich S, Murphy PA. Estrogenic activity of glycitein, a soy isoflavone. J Agric Food Chem. 1999 Apr;47(4):1607-10.

[3]Yoshida H, Teramoto T, Ikeda K, Yamori Y. Glycitein effect on suppressing the proliferation and stimulating the differentiation of osteoblastic MC3T3-E1 cells. Biosci Biotechnol Biochem. 2001;65(5):1211-1213

[4]Song TT, Hendrich S, Murphy PA. Estrogenic activity of glycitein, a soy isoflavone [published correction appears in J Agric Food Chem 2002 Apr 10;50(8):2470]. J Agric Food Chem. 1999;47(4):1607-1610

[5]Zhang B, Su JP, Bai Y, Li J, Liu YH. Inhibitory effects of O-methylated isoflavone glycitein on human breast cancer SKBR-3 cells. Int J Clin Exp Pathol. 2015;8(7):7809-7817. Published 2015 Jul 1

[6]Winzer M, Rauner M, Pietschmann P. Glycitein decreases the generation of murine osteoclasts and increases apoptosis. Wien Med Wochenschr. 2010;160(17-18):446-451