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Atractyloside potassium salt

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Chemical Structure| 102130-43-8 同义名 : 苍术苷二钾盐 ;Atractyloside (potassium salt);ATR potassium salt;Atractyloside Potassium
CAS号 : 102130-43-8
货号 : A230303
分子式 : C30H44K2O16S2
纯度 : 95%
分子量 : 802.987
MDL号 : MFCD00078810
存储条件：

粉末 Sealed in dry,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 250 mg/mL(311.34 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO

H2O: 65 mg/mL(80.95 mM)，配合低频超声助溶
动物实验配方：
生物活性
描述 The adenine nucleotide translocator (ANT) accomplishes the exchange of ATP from the mitochondrial matrix with cytoplasmic ADP[1].Atractyloside potassium salt is a toxic diterpenoid glycoside that can be isolated from the fruits of Xanthium sibiricum. Atractyloside potassium salt is a powerful and specific inhibitor of mitochondrial ADP/ATP transport. Atractyloside potassium salt inhibits chloride channels from mitochondrial membranes of rat heart[2].Upon cell-death induction, ANT1 becomes a substrate for type 2 transglutaminase(TG2)'s cross-linking activity and the lack of TG2 results in a reduction of apoptosis as well as in a marked sensitivity to the ADP/ATP exchange inhibition by atractyloside[3].Renal and hepatic pyruvate-stimulated gluconeogenesis were significantly (P < 0.05) inhibited at atractyloside concentrations of > or =500 microM. Accumulation of organic anion p-amino-hippuric acid (PAH) was also inhibited in renal cortical slices at atractyloside concentrations of > or =500 microM[4]. Atractyloside in large amounts gives rise to massive necrosis, but in vitro studies have shown that at lower doses cells progress to apoptosis[5].In vitro proximal tubular cells are selectively sensitive to atractyloside, whereas other renal cell types are quite resistant. There are also differences in the response of liver and renal tissue to atractyloside[6].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

1.25mL

0.25mL

0.12mL

6.23mL

1.25mL

0.62mL

12.45mL

2.49mL

1.25mL
参考文献

[1]Erika Cione,et al. Coenzyme A enhances activity of the mitochondrial adenine nucleotide translocator. Int J Biochem Cell Biol. 2010 Jan;42(1):106-12.

[2]Nikles S, et al. Influence of Processing on the Content of Toxic Carboxyatractyloside and Atractyloside and the Microbiological Status of Xanthium sibiricum Fruits (Cang'erzi). Planta Med. 2015 Aug;81(12-13):1213-20.

[3]W Malorni,et al. The adenine nucleotide translocator 1 acts as a type 2 transglutaminase substrate: implications for mitochondrial-dependent apoptosis. Cell Death Differ. 2009 Nov;16(11):1480-92.

[4] D K Obatomi,et al. The toxic mechanism and metabolic effects of atractyloside in precision-cut pig kidney and liver slices. Arch Toxicol. Jul-Aug 1998;72(8):524-30.

[5]M J Stewart,et al. The biochemistry and toxicity of atractyloside: a review. Ther Drug Monit. 2000 Dec;22(6):641-9.

[6] D K Obatomi,et al. Biochemistry and toxicology of the diterpenoid glycoside atractyloside. Food Chem Toxicol. 1998 Apr;36(4):335-46.