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MPTP HCl

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Chemical Structure| 23007-85-4 同义名 : MPTP hydrochloride
CAS号 : 23007-85-4
货号 : A211071
分子式 : C12H16ClN
纯度 : 99+%
分子量 : 209.715
MDL号 : MFCD00012790
存储条件：

粉末 Inert atmosphere,Room Temperature

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 12 mg/mL(57.22 mM)，配合低频超声，并水浴加热至45℃助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO

H2O: 100 mg/mL(476.84 mM)
动物实验配方：
生物活性
描述 Parkinson’s disease (PD) is characterized by the selective loss of dopaminergic neurons in the substantia nigra (SN). MPTP is a neurotoxin that can induce symptoms similar to those observed in PD in humans, nonhuman primates, and mice. In addition, MPTP induces selective loss of dopaminergic neurons in mice SN[3]. Mice in the MPTP group were intraperitoneally injected with 20 mg/kg MPTP hydrochloride dissolved in saline, with four injections at 3-hour intervals over a period of 1 day. The number of tyrosine hydroxylase-immunoreactive neurons in the MPTP group was significantly lower than in the normal group (P < 0.05), indicating successful establishment of the Parkinson's disease model. The changes in thioredoxin reductase 1 protein expression in the midbrain 7 days after the last injection of MPTP were detected. The MPTP-treated mice showed a 40% decrease in thioredoxin reductase 1 protein level compared with the normal mice (P < 0.05). Moreover, there was a pronounced reduction in thioredoxin reductase 1 mRNA level in MPTP-treated mice compared with normal mice (P < 0.05)[4].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

4.77mL

0.95mL

0.48mL

23.84mL

4.77mL

2.38mL

47.68mL

9.54mL

4.77mL
参考文献

[1]Liu Z, Jing Y, et al. Downregulation of thioredoxin reductase 1 expression in the substantia nigra pars compacta of Parkinson's disease mice. Neural Regen Res. 2013 Dec 15;8(35):3275-83.

[2]Nicotra A, Parvez S. Apoptotic molecules and MPTP-induced cell death. Neurotoxicol Teratol. 2002 Sep-Oct;24(5):599-605.

[3]Nicotra A, Parvez S. Apoptotic molecules and MPTP-induced cell death. Neurotoxicol Teratol. 2002 Sep-Oct;24(5):599-605. doi: 10.1016/s0892-0362(02)00213-1. PMID: 12200191.

[4]Liu Z, Jing Y, Yin J, Mu J, Yao T, Gao L. Downregulation of thioredoxin reductase 1 expression in the substantia nigra pars compacta of Parkinson's disease mice. Neural Regen Res. 2013 Dec 15;8(35):3275-83. doi: 10.3969/j.issn.1673-5374.2013.35.002. PMID: 25206649; PMCID: PMC4145943.