产品说明书

IWR-1

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Chemical Structure| 1127442-82-3 同义名 : IWR-1-endo;endo-IWR 1
CAS号 : 1127442-82-3
货号 : A205590
分子式 : C25H19N3O3
纯度 : 99%+
分子量 : 409.437
MDL号 : MFCD18086875
存储条件：

粉末 Sealed in dry,2-8°C

液体 -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 50 mg/mL(122.12 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：

IP 3% DMSO+2% Tween80+40% PEG300+water 1 mg/mL clear

PO 0.5% CMC-Na 45 mg/mL suspension

生物活性
描述 Although both IWR-1 and XAV939 act as reversible Wnt pathway inhibitors and exhibit similar pharmacological effects both in vitro and in vivo, IWR-1 exerts its effect via interaction with Axin, while XAV939 binds TNKS directly.
细胞研究
细胞系 浓度 检测类型 检测时间 活动说明 数据源
Escherichia coli BL21 (DE3) cells Function assay 90 mins Activity of N-terminus hexaHis-tagged human TNSK2 expressed in Escherichia coli BL21 (DE3) cells using biotinylated NAD+ as substrate after 90 mins by Western blot analysis, EC50=0.2 μM 22233320
HEK293 cells Function assay Displacement of IWR-PB from Axin2 in HEK293 cells by Western blot method 19125156
human DLD1 cells Function assay 2 h Induction of Axin2 stabilization in human DLD1 cells assessed as decreased transcription of Axin2 after 2 hrs by RT-PCR method. 19125156
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.44mL

0.49mL

0.24mL

12.21mL

2.44mL

1.22mL

24.42mL

4.88mL

2.44mL