产品说明书
FCCP
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同义名 : | Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone;Trifluoromethoxy carbonylcyanide phenylhydrazone;para-Trifluoromethoxyphenylhydrazone, Carbonyl Cyanide.;p-Trifluoromethoxyphenylhydrazone, Carbonyl Cyanide;Carbonyl Cyanide para Trifluoromethoxyphenylhydrazone;Carbonyl cyanide-p-trifluoromethoxyphenylhydrazone |
| CAS号 : | 370-86-5 | |
| 货号 : | A194814 | |
| 分子式 : | C10H5F3N4O | |
| 纯度 : | 99%+ | |
| 分子量 : | 254.168 | |
| MDL号 : | MFCD00009699 | |
| 存储条件： |
粉末 Keep in dark place,Inert atmosphere,Room temperature 液体 -20°C:3-6个月-80°C:12个月 |
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| 溶解度 : |
DMSO: 105 mg/mL(413.11 mM)，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO 无水乙醇: 30 mg/mL(118.03 mM)，注意：无水乙醇开封后，易挥发，也会吸收空气中的水分，导致溶解能力下降，请避免使用开封较久的乙醇 |
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| 动物实验配方： |
IP 2% DMSO+2% Tween80+40% PEG300+water 3 mg/mL clear PO 0.5% CMC-Na 36 mg/mL suspension |
| 生物活性 | |||
|---|---|---|---|
| 描述 | FCCP is a potent mitochondrial oxidative phosphorylation uncoupler which exhibits uncoupling effects at concentrations down to 10 -8 molar in mouse liver mitochondria[1]. Treatment with 5μM FCCP led to a reduction of ATP levels and ROS production and stimulated mitochondrial biogenesis related genes including Pgc-1α, mitochondrial genes such as Tfam and COXIV in hepatic stellate cells[2]. FCCP at concentration of 62.5nM and 125nM significantly reduced the mitochondrial membrane potential in the cytoplasmic and cortical region, as well as decreased ATP level of 8-cell embryos[3]. The processing of mutant APP in K695sw cells and wild-type APP in K695 cells was inhibited post treatment with 5μM FCCP for 4h[4]. | ||
| 作用机制 | FCCP is a protonophore which can induce ionic currents carried by Na+ and H+ and depolarization dependent on the plasmalemmal proton gradient.[5] | ||
| 细胞研究 | |||||
|---|---|---|---|---|---|
| 细胞系 | 浓度 | 检测类型 | 检测时间 | 活动说明 | 数据源 |
| Hep3B cells | 10 μM | Function assay | Stimulation of state 4 cellular respiration in human Hep3B cells at 10 uM in presence of oligomycin | 22938093 | |
| MDA-MB-231 cells | 1 uM | Cytotoxicity assay | Cytotoxicity against human MDA-MB-231 cells assessed as inhibition of cell proliferation/viability at 1 uM | 23245650 | |
| SH-SY5Y cells | 10 μM | Function assay | 10 mins | Induction of mitochondrial membrane potential loss in human SH-SY5Y cells at 10 uM incubated for 10 mins in presence of 0.2 uM CsA by TMRE dye based assay | 25265024 |
| 临床研究 | |||||
|---|---|---|---|---|---|
| NCT号 | 适应症或疾病 | 临床期 | 招募状态 | 预计完成时间 | 地点 |
| NCT00613860 | Emphysema COP... 展开 >>D 收起 << | Phase 3 | Unknown | December 2011 | Germany ... 展开 >> Charite, University of Berlin Recruiting Berlin, Germany, 10117 Contact: Christian Witt, MD, PhD +49 (030) 450 565 022 pneumologie@charite.de Contact: Bernd Schmitt, MD +49 (030) 450 565 02 pneumologie@charite.de Thoraxklinik, University of Heidelberg Recruiting Heidelberg, Germany, 69190 Principal Investigator: Felix JF Herth, MD, PhD Medical Center Nürnberg Recruiting Nürnberg, Germany, 90340 Contact: Joachim Ficker, MD, PHD +49 (911) 398-2675 ficker@klinikum-nuernberg.de Contact: Manfred Wagner, MD +49 (911) 398-0 Manfred.Wagner@klinikum-nuernberg.de 收起 << |
| NCT00176540 | Fatigue Unspe... 展开 >>cified Adult Solid Tumor, Protocol Specific Unspecified Childhood Solid Tumor, Protocol Specific 收起 << | Not Applicable | Terminated(slow accrual) | - | United States, New Jersey ... 展开 >> Cancer Institute of New Jersey at Hamilton Hamilton, New Jersey, United States, 08690 Cancer Institute of New Jersey at UMDNJ - Robert Wood Johnson Medical School New Brunswick, New Jersey, United States, 08903 收起 << |
| NCT01187147 | Asthma | Not Applicable | Completed | - | Hong Kong ... 展开 >> University of Hong Kong Queen Mary Hospital Hong Kong, Hong Kong, 0 收起 << |
| 实验方案 | |||
|---|---|---|---|
| 1mg | 5mg | 10mg | |
|
1 mM 5 mM 10 mM |
3.93mL 0.79mL 0.39mL |
19.67mL 3.93mL 1.97mL |
39.34mL 7.87mL 3.93mL |
| 参考文献 |
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