产品说明书
Dimethadione
 |
同义名 : | 二甲双酮 |
| CAS号 : | 695-53-4 | |
| 货号 : | A181810 | |
| 分子式 : | C5H7NO3 | |
| 纯度 : | 95% | |
| 分子量 : | 129.114 | |
| MDL号 : | MFCD00005379 | |
| 存储条件： |
粉末 Sealed in dry,2-8°C 液体 -20°C:3-6个月-80°C:12个月 |
|
| 溶解度 : |
DMSO: 105 mg/mL(813.23 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO |
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| 动物实验配方： |
| 生物活性 | |||
|---|---|---|---|
| 描述 | 5,5'-Dimethyloxazolidine-2,4-dione (DMO), a weak non-metabolizable acid, is commonly utilized for determining intracellular pH. In these studies, DMO was tested as an inducer of differentiation on the basis that its uptake and subsequent dissociation might transiently raise intracellular pH and activate ion-fluxes critical for triggering maturation. After 5 days of exposure to 40 mM DMO, greater than 60% of HL-60 cells displayed phenotypic and functional changes characteristic of mature granulocytes. DMO was either weak or inactive as an inducer of murine erythroleukemia cell (FLC) differentiation[2]. Determination of the overall internal pH of the cells, using (14)C-benzoic acid (BA) or DMO, showed that the cells maintain a neutral pH (6.6 to 7.2) over an external pH range of 3.0-7.0[3]. The internal pH of S. petersenii determined using (14)C-benzoic acid and [2-(14)C]-5,5-dimethyloxazolidine-2,4-dione was pH 7.0-7.5, over an external pH range of 5.0-7.5[4]. The glucose effect on DMO uptake was concentration-dependent, with half-maximal effect at about 4 mM-glucose and maximum effect at about 10 mM-glucose[5]. | ||
| 实验方案 | |||
|---|---|---|---|
| 1mg | 5mg | 10mg | |
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1 mM 5 mM 10 mM |
7.75mL 1.55mL 0.77mL |
38.73mL 7.75mL 3.87mL |
77.45mL 15.49mL 7.75mL |
| 参考文献 |
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